69079 Sigma-AldrichPerfect Protein™ Markers, 10-225 kDa
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Overview
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Pricing & Availability
| Catalog Number | Availability | Packaging | Qty/Pack | Price | Quantity | |
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| 69079-3 |
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Glass bottle | 100 lane |
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| Description | |
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| Overview | The Perfect Protein™ Markers are a novel set of recombinant proteins with defined
sizes at convenient intervals. Designed for routine use in SDS-polyacrylamide gel
electrophoresis, the Perfect Protein Markers enable highly accurate size determination of
unknown samples. Unlike many conventional markers (e.g., ovalbumin, serum albumin, etc.), the Perfect Protein Markers contain no oligosaccharides that cause anomalous migration, heterogeneous "fuzzy" bands, or inaccurate size estimation. The markers are optimized for use with Coomassie blue staining, but adjusted amounts can also be used with other gel staining methods (e.g., silver staining, fluorescent dyes, etc.). The Perfect Protein Markers, 15-150 kDa, include protein sizes of 15, 25, 35, 50, 75, 100, and 150 kDa. Each vial contains 400 µg protein. A 5-µl load contains 0.5 µg for each of the protein sizes, except for the 50-kDa band, which contains twice as much protein (1.0 µg). The Perfect Protein Markers, 10-225 kDa, include the protein sizes listed above and two additional proteins, 10 kDa and 225 kDa, for applications requiring a broader size range. Each vial contains 500 µg protein. A 5-µl load contains 0.5 µg for each of the protein sizes, except for the 50-kDa band, which contains twice as much protein (1.0 µg).  The 4X SDS Sample Buffer (Cat. No. 70607), which is separately available, is a standard formulation commonly used for SDS-PAGE analysis of proteins. The solution includes DTT for complete denaturation of disulfide bonds. The buffer can be used at a final concentration of 2X for most applications. |
| Catalogue Number | 69079 |
| Brand Family | Novagen® |
| Features and benefits | • Markers are supplied at the working dilution in
gel loading buffer; concentration optimized for
Coomassie blue staining • Recombinant, unmodified markers produce sharp, accurately sized bands • Available in two size ranges: 15-150 kDa 10-225 kDa |
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| Form | Liquid |
| Quality Level | MQ100 |
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| Ship Code | Blue Ice Only |
| Toxicity | Toxic |
| Storage | -20°C |
| Avoid freeze/thaw | Avoid freeze/thaw |
| Do not freeze | Ok to freeze |
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| Specifications |
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| Global Trade Item Number | |
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| Catalog Number | GTIN |
| 69079-3 | 07790788052515 |
Documentation
Perfect Protein™ Markers, 10-225 kDa Certificates of Analysis
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| 69079 |
Brochure
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| Protein Purification and Detection Tools |
Technical Info
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| Western Blotting Quick Reference Product Guide |