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70608 pETBlue™-1 DNA - Novagen

Novagen's pETBlue™-1 vector is designed to identify recombinants by traditional blue/white screening while also allowing T7lac promoter based expression of target genes.

pETBlue™-1 DNA - Novagen MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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70608
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Overview

Replacement Information

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      		 Plastic ampoule 20 μg
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      Description
      OverviewThe pETBlue™-1 vector is designed to identify recombinants by traditional blue/white screening while also allowing T7lac promoter based expression of target genes. Screening is independent of expression because the T7lac expression promoter is in an opposed orientation relative to the E. coli promoter that mediates blue/white screening. pETBlue-1 facilitates the expression of native unfused proteins and allows convenient subcloning of target genes already fused to existing detection and purification tags. The EcoR V cloning site is appropriately spaced downstream of an E. coli ribosome binding site. Inserts must encode an ATG start codon at their 5′ end if expression is desired. The sequence is numbered from the first base of the T7 promoter sequence.




      This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
      Catalogue Number70608
      Brand Family Novagen®
      References
      Product Information
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      70608-3 04055977257458

      Documentation

      pETBlue™-1 DNA - Novagen SDS

      Title

      Safety Data Sheet (SDS) 

      pETBlue™-1 DNA - Novagen Certificates of Analysis

      TitleLot Number
      70608

      Citations

      Title
    • Yefim Manevich, et al. (2007) Structure and phospholipase function of peroxiredoxin 6: Identification of the catalytic triad and its role in phospholipid substrate binding. Journal of Lipid Research 48, 2306-2318.
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      User Protocols

      Title
      TB053 Academic and Non-profit Laboratory Assurance Letter
      TB249 pETBlue™ System Manual

      Vector Map

      Title
      TB258VM pETBlue™-1 Vector Map