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382159 Sigma-AldrichPhosphoDetect™ Anti-Histone H3 (pSer¹⁰) (7-20) Rabbit pAb

This PhosphoDetect™ Anti-Histone H3 (pSer¹⁰) (7-20) Rabbit pAb is validated for use in Immunoblotting, ICC, IHC, IP for the detection of Histone H3 (pSer¹⁰) (7-20).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
References
Data Sheet

Recommended Products

Overview

Replacement Information

Key Specifications Table

Species Reactivity	Host	Antibody Type
Nem, Dr, H, T, Xn	Rb	Polyclonal Antibody

Pricing & Availability

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Description
Overview	Recognizes the ~17 kDa histone H3 protein phosphorylated at Ser¹⁰.
Catalogue Number	382159
Brand Family	Calbiochem®
Application Data	Detection of human histone H3, phosphorylated on Ser¹⁰, by immunoprecipitation. Sample: Acid extracted proteins from HeLa cells. Primary antibody: PhosphoDetect™ Anti-Histone H3 (pSer¹⁰) (7-20) Rabbit pAb (Cat. No. 382159) (assay dependent). Detection: chemiluminescence.

References
References	Ajiro, K., et al. 1996. J. Biol. Chem. 271, 13197. Chadee, D.N., et al. 1995. J. Biol. Chem. 270, 20098. Mahadevan, L.C., et al. 1991. Cell 65, 775.

Product Information
Form	Liquid
Formulation	In 150 mM NaCl, 100 mM Tris-glycine, pH 7.4.
Positive control	Acid-extracted proteins from colcemid-arrested HeLa cells
Preservative	≤0.1% sodium azide
Quality Level	MQ100

Applications
Key Applications	Immunoblotting (Western Blotting) Immunocytochemistry Immunohistochemistry Immunoprecipitation
Application Notes	Immunoblotting (0.5 µg/ml) Immunocytochemistry (5 µg/ml) Immunohistochemistry (see comments) Immunoprecipitation (4 µg/10 µg acid-extracted proteins from colcemid-arrested HeLa cells)
Application Comments	For immunocytochemistry, colcemid-arrested HeLa cells were fixed with 1% paraformaldehyde. Also detects histone H3 in Tetrahymena micronuclei. The antibody has been reported to detect mitotic cells in Xenopus, Caenorhabditis elegans, and Drosophila embryos. Does not cross-react with unphosphorylated histone H3. This antibody has also been reported to work for immunohistochemistry. Variables associated with assay conditions will dictate the optimal working dilution.

Biological Information
Immunogen	a synthetic phosphopeptide [ARKpSTGGKAPRKQL(C)] corresponding to amino acids 7-20 surrounding the Ser¹⁰ phosphorylation site of human histone H3, conjugated to KLH
Immunogen	Human
Host	Rabbit
Isotype	IgG
Species Reactivity	Nematode Drosophila Human Tetrahymena Xenopus
Antibody Type	Polyclonal Antibody
Concentration Label	Please refer to vial label for lot-specific concentration

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Dry Ice Only
Toxicity	Standard Handling
Storage	-20°C
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalog Number	GTIN
382159-50UG	04055977213218

Documentation

PhosphoDetect™ Anti-Histone H3 (pSer¹⁰) (7-20) Rabbit pAb SDS

Title
Safety Data Sheet (SDS)

PhosphoDetect™ Anti-Histone H3 (pSer¹⁰) (7-20) Rabbit pAb Certificates of Analysis

Title	Lot Number
382159	Enter Lot Number

References

Reference overview
Ajiro, K., et al. 1996. J. Biol. Chem. 271, 13197. Chadee, D.N., et al. 1995. J. Biol. Chem. 270, 20098. Mahadevan, L.C., et al. 1991. Cell 65, 775.

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	02-August-2007 RFH
Application	Immunoblotting (0.5 µg/ml) Immunocytochemistry (5 µg/ml) Immunohistochemistry (see comments) Immunoprecipitation (4 µg/10 µg acid-extracted proteins from colcemid-arrested HeLa cells)
Application Data	Detection of human histone H3, phosphorylated on Ser¹⁰, by immunoprecipitation. Sample: Acid extracted proteins from HeLa cells. Primary antibody: PhosphoDetect™ Anti-Histone H3 (pSer¹⁰) (7-20) Rabbit pAb (Cat. No. 382159) (assay dependent). Detection: chemiluminescence.
Description	Protein A purified rabbit polyclonal antibody. Recognizes the ~17 kDa histone H3 protein phosphorylated at Ser¹⁰.
Background	Histone H1 and H3 phosphorylations correlate with the process of chromosome condensation. H3 mRNA-positive cells are most often found in tissues with a high proliferation rate, such as basal cells of the skin and crypt cells of the intestine.
Host	Rabbit
Immunogen species	Human
Immunogen	a synthetic phosphopeptide [ARKpSTGGKAPRKQL(C)] corresponding to amino acids 7-20 surrounding the Ser¹⁰ phosphorylation site of human histone H3, conjugated to KLH
Isotype	IgG
Species	C. elegans, Drosophila, Tetrahymena, Xenopus, human
Positive control	Acid-extracted proteins from colcemid-arrested HeLa cells
Form	Liquid
Formulation	In 150 mM NaCl, 100 mM Tris-glycine, pH 7.4.
Concentration Label	Please refer to vial label for lot-specific concentration
Preservative	≤0.1% sodium azide
Comments	For immunocytochemistry, colcemid-arrested HeLa cells were fixed with 1% paraformaldehyde. Also detects histone H3 in Tetrahymena micronuclei. The antibody has been reported to detect mitotic cells in Xenopus, Caenorhabditis elegans, and Drosophila embryos. Does not cross-react with unphosphorylated histone H3. This antibody has also been reported to work for immunohistochemistry. Variables associated with assay conditions will dictate the optimal working dilution.
Storage	Avoid freeze/thaw -20°C
Do Not Freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).
Toxicity	Standard Handling
References	Ajiro, K., et al. 1996. J. Biol. Chem. 271, 13197. Chadee, D.N., et al. 1995. J. Biol. Chem. 270, 20098. Mahadevan, L.C., et al. 1991. Cell 65, 775.

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