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AC111P Sheep Anti-Mouse IgG Antibody, Species absorbed Antibody, HRP conjugate

Sheep Anti-Mouse IgG, Species absorbed, HRP conjugate, Cat. No. AC111P, is a secondary antibody HRP conjugate, validated for use in Western Blotting application with little or no cross-reactivity toward mouse IgA & IgM, chicken IgY or IgG from human, rat, rabbit, guinea pig, and hamster species.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Species Reactivity: Mouse
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AC111P
500 µL  
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      Overview

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      Description
      Catalogue NumberAC111P
      ReplacesAP300P
      DescriptionSheep Anti-Mouse IgG Antibody, Species absorbed Antibody, HRP conjugate
      Background InformationImmunoglobulin G (IgG) is part of the family of glycoprotein immunoglobulins which stage immune responses against pathogenic organisms. IgG contributes to the immune response by mobilizing innate immune effector cells and inducing and pro-inflammatory responses. Such proinflammatory responses are mediated by Fc receptors which interact with sialylated Fc fragments on IgG molecules.
      References
      Product Information
      FormatHRP
      PresentationPurified sheep polyclonal antibody in PBS with StabilZyme® HRP Conjugate Stabilizer and 0.05% Proclin.
      Quality LevelMQ100
      Applications
      ApplicationSheep Anti-Mouse IgG, Species absorbed, HRP conjugate, Cat. No. AC111P, is a secondary antibody HRP conjugate, validated for use in Western Blotting application with little or no cross-reactivity toward mouse IgA & IgM, chicken IgY or IgG from human, rat, rabbit, guinea pig, and hamster species.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: Performance of a representative lot was validated at a 1:4,000 dilution for target band labeling following a mouse monoclonal GAPDH antibody (Cat. No. MAB374) in Western blotting application using 10 µg of HeLa cell lysate.
      Biological Information
      ImmunogenPurified mouse IgG.
      ConcentrationPlease refer to lot specific datasheet.
      HostSheep
      SpecificityBased on ELISA analysis using the unconjugated antibody (Cat. No. AC111), this antibody reacts with whole molecule mouse IgG (IgG2>IgG2a>IgG3>>>IgG1) with minimal or no cross-reactivity toward mouse IgA or IgM.
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse. This antibody has been species absorbed and tested by ELISA prior to conjugation to ensure minimal cross-reaction toward chicken IgY or IgG from human, rat, rabbit, guinea pig, and hamster species, but may cross-react with immunoglobulins from other species.
      Antibody TypePolyclonal Antibody
      Purification MethodAmmonium Sulfate.
      Molecular Weight~150.0 kDa observed.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in A431 cell lysate.

      Western Blotting Analysis: Performance of this HRP-conjugated secondary antibody (Cat No. AC111P) is validated at a 1:4,000 dilution for target band labeling following a mouse monoclonal GAPDH antibody (Cat. No. MAB374) in Western blotting application using 10 µg of A431 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size500 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      AC111P 04054839052934

      Documentation

      Sheep Anti-Mouse IgG Antibody, Species absorbed Antibody, HRP conjugate SDS

      Title

      Safety Data Sheet (SDS) 

      Sheep Anti-Mouse IgG Antibody, Species absorbed Antibody, HRP conjugate Certificates of Analysis

      TitleLot Number
      Sheep Anti-Mouse IgG, Species absorbed, HRP conjugate - 3474111 3474111
      Sheep Anti-Mouse IgG, Species absorbed, HRP conjugate - 3596228 3596228
      Sheep Anti-Mouse IgG, Species absorbed, HRP conjugate - 3749948 3749948
      Sheep Anti-Mouse IgG, Species absorbed, HRP conjugate - 3878279 3878279
      Sheep Anti-Mouse IgG, Species absorbed, HRP conjugate - 4080463 4080463
      Sheep Anti-Mouse IgG, Species absorbed, HRP conjugate -Q2735581 Q2735581
      Sheep Anti-Mouse IgG, Species absorbed, HRP conjugate Polyclonal Antibody 3153211
      Sheep Anti-Mouse IgG, Species absorbed, HRP conjugate Polyclonal Antibody 2981025