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109215 Ziehl-Neelsen carbol-fuchsin solution

for microscopy

Ziehl-Neelsen carbol-fuchsin solution MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Carbol fuchsin solution

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109215
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      		 Glass bottle 100 ml
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      1092150507
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          		 Plastic bottle 500 ml
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          1092152507
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              		 Plastic bottle 2.5 l
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              Description
              Catalogue Number109215
              SynonymsCarbol fuchsin solution
              DescriptionZiehl-Neelsen carbol-fuchsin solution
              OverviewZiehl-Neelsen carbolfuchsin solution - for microscopy, is a ready-to use solution used in the Ziehl-Neelsen hot staining for the microscopic investigation of acid-fast bacteria(AFB). The cell wall of acid-fast bacteria has a high proportion of wax and lipids and hence absorbs dyes only very slowly. The most efficient staining method is the hot staining according to Ziehl-Neelsen. In this method, carbolfuchsin solution is applied to the specimen and heated. This heating process accelerates the rate at which the fuchsin dye is absorbed and thus also that of the formation of the mycolate-fuchsin complex in the cell wall. Once the acid-fast bacteria have absorbed the fuchsin dye, it is virtually impossible to decolorize them again, even when they are intensively treated with a decolorizing solution such as e. g. hydrochloric acid in ethanol (Cat. no. 100327). Accordingly, acid-fast bacteria are termed as acid- and alcohol-fast for staining, and are stained red in the microscopic visualization. Correspondingly, all non-acid-fast microorganisms are counterstained with an appropriate dye. In this instruction for use Löfföer's methylene blue solution(Cat. no. 101287) is used for counterstaining. Those three solutions are each 500 ml and are contained in the AFB-color modified staining kit (Cat. No.100497).
              The 500 ml bottle of Ziehl-Neelsen carbolfuchsin solution provides approx. 250 stainings. Pretreatment of the specimens with Sputofluol® dissolves the bacteria from the surrounding viscid sputum and cell material. Ziehl-Neelsen carbolfuchsin solution - for microscopy is an IVD product and CE registered. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
              References
              Product Information
              HS Code3212 90 90
              Quality LevelMQ400
              Applications
              Applicationfor microscopy
              Biological Information
              Physicochemical Information
              Density0.99 g/cm3 (20 °C)
              Flash point47 °C
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              Hazard Pictogram(s) Hazard Pictogram(s)
              Hazard Pictogram(s)
              Hazard Pictogram(s)
              Hazard Pictogram(s)
              Hazard Statement(s)H226: Flammable liquid and vapour.
              H314: Causes severe skin burns and eye damage.
              H341: Suspected of causing genetic defects.
              H410: Very toxic to aquatic life with long lasting effects.
              Precautionary Statement(s)P210: Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
              P273: Avoid release to the environment.
              P280: Wear protective gloves/ protective clothing/ eye protection/ face protection/ hearing protection.
              P303 + P361 + P353: IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water.
              P304 + P340 + P310: IF INHALED: Remove person to fresh air and keep comfortable for breathing. Immediately call a POISON CENTER/doctor.
              P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
              Signal WordDanger
              Storage class3 Flammable liquids
              WGKWGK 3 highly hazardous to water
              Disposal9
              Carcinogenic compounds and flammable compounds labelled "Highly toxic" or "Toxic": container F. Alkyl sulfates are carcinogenic; Take particular care to avoid inhalation and skin contact. To neutralize alkyl sulfates, add dropwise (from a dropping funnel) to concentrated ice-cool Ammonia solution (Cat.No. 105426) with vigorous stirring. Before placing in container D, check the pH with pH Universal indicator strips (Cat.No. 109535).
              Safety Information
              Categories of dangerflammable, harmful, corrosive, mutagenic
              Product Usage Statements
              Storage and Shipping Information
              StorageStore at +15°C to +25°C.
              Packaging Information
              Transport Information
              Declaration (railroad and road) ADR, RIDUN 1992 , 3 (6.1), III
              Declaration (transport by air) IATA-DGRUN 1992 , 3 (6.1), III
              Declaration (transport by sea) IMDG-CodeUN 1992 , 3 (6.1), III
              Supplemental Information
              Specifications
              Global Trade Item Number
              Catalog Number GTIN
              1092150107 04054839131936
              1092150507 04054839116780
              1092152507 04054839120282

              Related Products & Applications

              Accessories (Primary)

              Catalog Number Description  
              100327 Hydrochloric acid in ethanol Show Pricing & Availability
              101287 Löffler's methylene blue solution Show Pricing & Availability

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              Categories

              IVD/OEM Materials & Reagents > Microscopy > Bacteriology > Auxiliaries for Bacteriology
              IVD/OEM Materials & Reagents
              IVD/OEM Materials & Reagents > Microscopy > Bacteriology > General Bacteriological Staining Solutions
              IVD/OEM Materials & Reagents > Microscopy > Bacteriology > Acid-Fast Bacteria Staining