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MABE1045 Anti-CTIP2/BCL11B Antibody, clone 25B6

Anti-CTIP2/BCL11B Antibody, clone 25B6, Cat. No. MABE1045, is a highly specific rat monoclonal antibody that targets CTIP2 and has been tested in Immunofluorescence, Immunohistochemistry, and Western Blotting.

Anti-CTIP2/BCL11B Antibody, clone 25B6 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: Bcl11b, Ctip2, Rit1 UniProt Number: Q99PV8
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MABE1045
100 μg  
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      Overview

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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, HWB, IH(P)RPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE1045
      DescriptionAnti-CTIP2/BCL11B Antibody, clone 25B6
      Alternate Names
      • B-cell lymphoma/leukemia 11B
      • B-cell CLL/lymphoma 11B
      • BCL-11B
      • COUP-TF-interacting protein 2
      • mRit1
      • Radiation-induced tumor suppressor gene 1 protein
      Background InformationB-cell lymphoma/leukemia 11B (UniProt Q99PV8; also known as B-cell CLL/lymphoma 11B, BCL-11B, COUP-TF-interacting protein 2, mRit1, Radiation-induced tumor suppressor gene 1 protein) is encoded by the Bcl11b (also known as Ctip2, Rit1) gene (Gene ID 58208) in murine species. CTIP2/CBL11B is a C2H2 zinc finger protein that binds GC-rich response elements and is reported to associate with a variety of cofactors, including the corepressor complexes NuRD, Sirt1, histone acetyltransferase (HAT) p300, functioning both as a transcriptional repressor and activator. CTIP2/CBL11B is required at multiple developmental checkpoints for T cell commitment and maintenance. CTIP2/CBL11B expression is initiated at double-negative 2 (DN2) stage of T cell development and is maintained in mature T lymphocytes, with lower levels found in NK cells, but not in B cells, myeloid or dendritic cells. Bcl11b-/- mice die shortly after birth due to a blockade of thymocyte development at the DN2 stage. Bcl11b-deficient DN2 thymocytes are highly proliferative and retain the potential to differentiate into NK cells and myeloid lineages, but not mature T-cells.
      References
      Product Information
      FormatPurified
      HS Code3002 15 90
      PresentationPurified rat monoclonal antibody IgG2a in buffer containing 150 mM NaCl, 15 mM HEPES, pH 7.5, with 0.02% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-CTIP2/BCL11B Antibody, clone 25B6, Cat. No. MABE1045, is a highly specific rat monoclonal antibody that targets CTIP2 and has been tested in Immunofluorescence, Immunohistochemistry, and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:240 dilution from a representative lot detected CTIP2/BCL11B in mouse brain tissue sections.

      Immunofluorescence Analysis: A representative lot detected CTIP2 localization in 4% paraformaldehyde-fixed floating embryonic (E14 & E16) and postnatal (P3 & P6) mouse brain sections (Arlotta, T., et al. (2005). Neuron. 45(2):207-221).

      Western Blotting Analysis: A representative lot detected doublet CTIP2 bands, representing two spliced isoforms, in Jurkat nuclear extracts as well as in SIRT1 immunoprecipitate (Senawong, T., et al. (2003). J. Biol. Chem. 278(44):43041-43050).
      Biological Information
      ImmunogenGST-tagged recombinant full-length mouse CTIP2 Beta isoform (Senawong, T., et al. (2003). J. Biol. Chem. 278(44):43041-43050).
      Clone25B6
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityClone 25B6 recognizes both the Alpha and Beta spliced isoforms of human and mouse CTIP2/BCL11B.
      IsotypeIgG2a
      Species Reactivity
      • Mouse
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Bcl11b
      • Ctip2
      • Rit1
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight~96 kDa observed. 95.52/94.57 kDa (human/mouse isoform Alpha) and 88.48/87.34 kDa (human/mouse isoform Beta) calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected CTIP2/BCL11B in 10 µg of HEK293 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABE1045 04054839049705

      Documentation

      Anti-CTIP2/BCL11B Antibody, clone 25B6 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-CTIP2/BCL11B Antibody, clone 25B6 Certificates of Analysis

      TitleLot Number
      Anti-CTIP2/BCL11B, clone 25B6 3068063
      Anti-CTIP2/BCL11B, clone 25B6 - 3234516 3234516
      Anti-CTIP2/BCL11B, clone 25B6 - 3459243 3459243
      Anti-CTIP2/BCL11B, clone 25B6 - 3596736 3596736
      Anti-CTIP2/BCL11B, clone 25B6 - 3996380 3996380
      Anti-CTIP2/BCL11B, clone 25B6 - 4044212 4044212
      Anti-CTIP2/BCL11B, clone 25B6 - 4123392 4123392
      Anti-CTIP2/BCL11B, clone 25B6 - 4139151 4139151
      Anti-CTIP2/BCL11B, clone 25B6 -Q2736875 Q2736875
      Anti-CTIP2/BCL11B, clone 25B6 Monoclonal Antibody 3009974