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MABT824 Anti-gamma-Actin/ACTG1 Antibody, clone 2A3

This Anti-gamma-Actin/ACTG1 Antibody, clone 2A3 is validated for use in Western Blotting, Immunocytochemistry for the detection of ACTG1.

Anti-gamma-Actin/ACTG1 Antibody, clone 2A3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Cytoskeleton Gene Symbol: ACTG1, ACTG, BRWS2, DFNA20, DFNA26 UniProt Number: P63261
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MABT824
100 μg  
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      Overview

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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, H, Ch, RWB, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT824
      DescriptionAnti-gamma-Actin/ACTG1 Antibody, clone 2A3
      Alternate Names
      • Actin, cytoplasmic 2
      • gamma-Actin
      Background InformationActin, cytoplasmic 2 (UniProt P63261; also known as Gamma-actin) is encoded by the ACTG1 (also known as ACTG, BRWS2, DFNA20, DFNA26) gene (Gene ID 71) in human. Actins are globular multi-functional proteins that serve as the basic building blocks of cytoskeletal microfilaments and are among the most conserved eukaryotic proteins. Six actin types exist, skeletal muscle alpha-actin is encoded by the ACTA1 gene, smooth muscle alpha-actin by the ACTA2 gene, cytoplasmic beta-actin by the ACTB gene, cardiac muscle alpha-actin by the ACTC1 gene, cytoplasmic gamma-actin by the ACTG1 gene, and smooth muscle gamma-actin by the ACTG2 (a.k.a. ACTA3) gene. Although actins show >90% overall sequence homology, isoforms do show spatial, temporal, and tissue-specific expression patterns and only 50-60% homology is found in their 18 N-terminal residues. Cytoplasmic β and γ-actins, are thought to be present in all cells, while the other four actin isoforms are typically found in specific adult muscle tissue types. Actins exist in a variety of structural states, depending on the specific ionic conditions or the interaction with ligand proteins. The oligomeric and polymeric forms that actin molecules assume are dependent on the distinct conformations they adopt.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-gamma-Actin/ACTG1 Antibody, clone 2A3 is validated for use in Western Blotting, Immunocytochemistry for the detection of ACTG1.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesWestern Blotting Analysis: 0.5 µg/mL from a representative lot detected gamma-Actin/ACTG1 in 10 µg of NIH/3T3 cell lysate.
      Immunocytochemistry Analysis: 5.0 µg/mL from a representative lot detected gamma-Actin/ACTG1 in HUVECs, HeLa and A431 cells.
      Western Blotting Analysis: A representative lot detected downregulated cytoplasmic gamma-actin levels in stimulated (by A23187, TRAP-6, TNF, LPS, or IFN-γ) human cerebral microvascular endothelial D3 cells (hCMEC/D3) and their microparticles (MPs) when compared with unstimulated hCMEC/D3 and their MPs (Latham, S.L., et al. (2013). FASEB J. 27(2):672-683).
      Western Blotting Analysis: A representative lot detected siRNA-mediated downregulation of cytoplasmic gamma-actin in A549 human lung carcinoma cells (Miazza, V., et al. (2011). Virology. 410(1):7-16).
      Western Blotting Analysis: A representative lot detected cytoplasmic gamma-actin, but not beta-actin separated by 2-D gel electrophoresis of purified chicken gizzard actins or total protein extracts from human subcutaneous fibroblasts (HSCFs), canine MDCK cells, and rat aorta tissue (Dugina, V., et al. (2009). J. Cell Sci. 122(Pt 16):2980-2988).
      Western Blotting Analysis: A representative lot detected BSA conjugated with cytoplasmic gamma-actin N-terminal peptide, but not BSA conjugated with N-terminal peptides derived from the 5 other actin types (Dugina, V., et al. (2009). J. Cell Sci. 122(Pt 16):2980-2988).
      Immunocytochemistry Analysis: A representative lot detected TNF-stimulated localization of γ-actin into thick, intensely staining stress fibers concentrated apically in a submembranous network in human cerebral microvascular endothelial D3 cells (hCMEC/D3) (Latham, S.L., et al. (2013). FASEB J. 27(2):672-683).
      Immunocytochemistry Analysis: A representative lot detected similar γ-actin cytoskeleton structure in human cerebral microvascular endothelial D3 cells (hCMEC/D3) with or without Rho kinase inhibitor Y-27632 treatment (Latham, S.L., et al. (2013). FASEB J. 27(2):672-683).
      Immunocytochemistry Analysis: A representative lot detected a drastic subcellular redistribution of gamma-actin following Sendai virus infection of polarized Madin-Darby canine kidney (MDCK) epithelial cells by fluorescent immunocytochemistry staining of paraformaldehyde-fixed, methanol-treated cells (Miazza, V., et al. (2011). Virology. 410(1):7-16).
      Immunocytochemistry Analysis: A representative lot detected cytoplasmic gamma-actin subcellular localization distinct from that of beta-actin in both spreading and stationary cells by fluorescent immunocytochemistry, using paraformaldehyde-fixed, methanol-treated HSCF human subcutaneous fibroblasts, HaCaT human keratinocytes, WI38 human embryonic fibroblasts,and Madin-Darby canine kidney (MDCK) cells (Dugina, V., et al. (2009). J. Cell Sci. 122(Pt 16):2980-2988).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the the N-terminal sequence of human gamma-Actin/ACTG1.
      EpitopeNear N-terminus.
      Clone2A3
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 2A3 detected BSA conjugated with cytoplasmic gamma-actin N-terminal peptide, but not BSA conjugated with N-terminal peptides derived from the other 5 actin types (Dugina, V., et al. (2009). J. Cell Sci. 122(Pt 16):2980-2988).
      IsotypeIgG2bκ
      Species Reactivity
      • Mouse
      • Human
      • Chicken
      • Rat
      Species Reactivity NoteMouse, Human, Chicken, Rat. Predicted to react with all Mammals based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ACTG1
      • ACTG
      • BRWS2
      • DFNA20
      • DFNA26
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~39-45 kDa observed. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected gamma-Actin/ACTG1 in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABT824 04055977331165

      Documentation

      Anti-gamma-Actin/ACTG1 Antibody, clone 2A3 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-gamma-Actin/ACTG1 Antibody, clone 2A3 Certificates of Analysis

      TitleLot Number
      Anti-gamma-Actin/ACTG1, clone 2A3 3075584
      Anti-gamma-Actin/ACTG1, clone 2A3 - 3249915 3249915
      Anti-gamma-Actin/ACTG1, clone 2A3 - 3419586 3419586
      Anti-gamma-Actin/ACTG1, clone 2A3 - 3709486 3709486
      Anti-gamma-Actin/ACTG1, clone 2A3 - 3954773 3954773
      Anti-gamma-Actin/ACTG1, clone 2A3 - 4203374 4203374
      Anti-gamma-Actin/ACTG1, clone 2A3 -Q2653599 Q2653599

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies