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MABN92-25UG Anti-lba1/AIF1 (mouse monoclonal)

Anti-Iba1/AIF1 Antibody is an antibody against Iba1/AIF1 for use in WB, IH.

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MABN92-25UG
25 μg  
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      Description
      Catalogue NumberMABN92-25UG
      DescriptionAnti-lba1/AIF1 (mouse monoclonal)
      Alternate Names
      • Allograft inflammatory factor 1
      • AIF-1
      • Ionized calcium-binding adapter molecule 1
      • Protein G1
      Background InformationThe allograft inflammatory factor-1 (AIF-1) is a 17 kDa interferon-gamma (IFN-gamma) inducible Ca(2+)-binding EF-hand protein that is encoded within the HLA class III genomic region. AIF-1 is known to be identical with ionized Ca(2+)-binding adaptor 1, microglia response factor-1, and daintain. AIF-1 expression is mostly limited to the macrophage/monocyte lineage, and is augmented by cytokines, such as IFN-gamma. It is reported to be involved in neuronal function, inflammatory responses, allograft rejection, autoimmunity, and the activation and function of macrophages. It is also shown to be expressed in macrophages and microglial cells in autominnume disesases such as experimental autoimmune encephalomyelitis, and neuritis and uveitis models.
      References
      Product Information
      FormatPurified
      Control
      • Human thymus tissue lysate
      PresentationPurified mouse monoclonal IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Iba1/AIF1 Antibody is an antibody against Iba1/AIF1 for use in WB, IH.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesWestern Blot Analysis: 1 µg/mL from a representative lot detected Iba1/AIF1 in 10 µg of mouse thymus tissue lysate.

      Western Blot Analysis: 1 µg/mL from a representative lot detected Iba1/AIF1 in 10 µg of rat thymus tissue lysate.

      Immunohistochemistry Analysis: A 1:300 dilution from a representative lot detected Iba/AIF1 in human spleen tissue.
      Immunohistochemistry Analysis: A 1:1000 dilution from a representative lot detected Iba/AIF1 in rat spleen tissue
      Biological Information
      ImmunogenLinear peptide corresponding to human Iba1/AIF1.
      Clone20A12.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene is induced by cytokines and interferon. Its protein product is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. Three transcript variants encoding different isoforms have been found for this gene.
      Gene Symbol
      • AIF1
      • G1
      • IBA1
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.

      SUBUNIT STRUCTURE: Homodimer. Monomer. Interacts with LCP1.

      SUBCELLULAR LOCATION: Cytoplasm > cytoskeleton. Cell projection > ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Note: Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis.

      TISSUE SPECIFICITY: Detected in T-lymphocytes and peripheral blood mononuclear cells.
      Molecular Weight~17 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated in Western Blot in human thymus tissue lysate.

      Western Blot Analysis: 1 µg/mL of this antibody detected Iba1/AIF1 in 10 µg of human thymus tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABN92-25UG 04054839342769

      Documentation

      Anti-lba1/AIF1 (mouse monoclonal) Certificates of Analysis

      TitleLot Number
      Anti-Rhodopsin, clone 4D2 - 3313746 3313746
      Anti-lba1/AIF1 (mouse monoclonal) - 3312992 3312992
      Anti-lba1/AIF1 (mouse monoclonal) - 3478259 3478259
      Anti-lba1/AIF1 (mouse monoclonal) - 3736631 3736631
      Anti-lba1/AIF1 (mouse monoclonal) - 3800109 3800109
      Anti-lba1/AIF1 (mouse monoclonal) - 3814317 3814317
      Anti-lba1/AIF1 (mouse monoclonal) - 3866800 3866800
      Anti-lba1/AIF1 (mouse monoclonal) - 3942763 3942763
      Anti-lba1/AIF1 (mouse monoclonal) - 3978860 3978860
      Anti-lba1/AIF1 (mouse monoclonal) - 4046558 4046558
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