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ABS2095-25UG Anti-phospho RasGRP2 (Ser554)

Anti-phospho RasGRP2 (Ser554), Cat. No. ABS2095, is a rabbit polyclonal antibody that detects RAS guanyl-releasing protein 2 phosphorylated on Ser554 and has been tested for use in Western Blotting.

Anti-phospho RasGRP2 (Ser554) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: Rasgrp2 UniProt Number: Q9QUG9
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ABS2095-25UG
25 μg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, MkWBRbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberABS2095-25UG
      DescriptionAnti-phospho RasGRP2 (Ser554)
      Alternate Names
      • RAS guanyl-releasing protein 2
      • Calcium and DAG-regulated guanine nucleotide exchange factor I
      • CalDAG-GEFI
      • F25B3.3 kinase-like protein
      Background InformationRAS guanyl-releasing protein 2 (UniProt: Q9QUG9; also known as Calcium and DAG-regulated guanine nucleotide exchange factor I, RasGRP2, CalDAG-GEFI, F25B3.3 kinase-like protein) is encoded by the Rasgrp2 gene (Gene ID: 19395) in murine species. RasGRP proteins are considered as activators of Ras and other related small GTPases by virtue of functioning as guanine nucleotide exchange factors (GEFs). RasGRP-1 through -4 share many structural domains, however, they display subtle differences between each of the different family members. They are expressed in distinct patterns, such as in different cells of the hematopoietic system and in the brain. RasGRP2's expression is restricted to platelets and their precursors, megakaryocytes, and neutrophils. RasGRP2 functions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating Rap through the exchange of bound GDP for GTP. It may also activate other GTPases such as RRAS, RRAS2, NRAS, KRAS, but not HRAS. It is also reported to function in aggregation of platelets and adhesion of T-lymphocytes and neutrophils, probably through inside-out integrin activation. RaGRP2 is found both in the cytosol and associated with membranes. It is shown to be enriched at juxtamembrane areas and membrane ruffles. Three isoforms of RasGRP2 have been reported that are generated by alternative splicing. It contains two EF-hand domains, one N-terminal Ras-GEF domain, one phorbol-ester/DAG-type zinc finger domain, and one Ras-GEF domain. RasGRP2 deficient animals exhibit bleeding problems due to defects in platelets aggregation and degranulation. (Ref.: Olga Ksionda, O et al. (2013). Front Biol (Beijing). 8(5): 508 532).
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine, 10% Ethylene Glycol, pH 7.6 with 0.05% sodium azide.
      Applications
      ApplicationAnti-phospho RasGRP2 (Ser554), Cat. No. ABS2095, is a rabbit polyclonal antibody that detects RAS guanyl-releasing protein 2 phosphorylated on Ser554 and has been tested for use in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected phospho RasGRP2 (pSer554) in a kinase assay with GST-Rasgrp2 (amino acids 1-374) and PKA, Forskolin and SKF81279 stimulated mouse striatal muscle slices, COS-7 cells transfected with EGFP-Rasgrp2 and Myc-PKA, nucleus accumbens (NAc) from mice treated with cocaine, Nucleus accu (Nagai, T., et. al. (2016). Neuron. 89(3):550-65).
      Western Blotting Analysis: A representative lot detected RAS guanyl-releasing protein 2 purified from GST-Rasgrp2 transfected COS-7 cells and phosphorylated with GST-PKA catalytic subunit alpha, Forskolin stimulated mouse striatal muscle slices ( Courtesy of Dr. Kozo Kaibuchi at Nagoya University, Japan).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 11 amino acids surrounding Ser554 from the C-termnal region of murine RAS guanyl-releasing protein 2.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects RAS guanyl-releasing protein 2 phosphorylated on Ser554 in various mammalian species. It targets an epitope within 11 amino acids surrounding Ser554 from the C-terminal region.
      IsotypeIgG
      Species Reactivity
      • Mouse
      • Monkey
      Species Reactivity NoteMouse, Monkey. Predicted to react with Baboon, Human, Rat, Rabbit, Porcine based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Gene Symbol
      • Rasgrp2
      Modifications
      • Phosphorylation
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~90 kDa observed; 69.45 kda calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting with RAS guanyl-releasing protein 2 purified from GST-RasGRP2 transfected COS-7 cells and phosphorylated on Ser554 by GST-PKA calalytic subunit alpha (PKAc alpha).

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected phospho RasGRP2 (Ser554) purified from GST-RasGRP2 transfected COS-7 cells and phosphorylated on Ser554 by GST-PKA catalytic subunit alpha (PKAc alpha).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      ABS2095-25UG 04054839505560