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05-421-AF488 Anti-PP2A, C subunit Antibody, clone 1D6, Alexa Fluor® 488 Conjugate

Anti-PP2A, C subunit Antibody, clone 1D6, Alexa Fluor® 488 Conjugate is an antibody against PP2A for use in Immunocytochemistry.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Kinases & Phosphatases Gene Symbol: PPP2CA, PP2CA UniProt Number: P67775
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05-421-AF488
100 µL  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, Yeast, Xn, B, R, Rb, MICCMAlexaFluor®488Monoclonal Antibody
      Description
      Catalogue Number05-421-AF488
      DescriptionAnti-PP2A, C subunit Antibody, clone 1D6, Alexa Fluor® 488 Conjugate
      Alternate Names
      • Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
      • PP2A-alpha
      • PP2Ac
      • PP2Calpha
      • Replication protein C
      • RP-C
      Background InformationSerine/threonine-protein phosphatase 2A catalytic subunit alpha isoform (EC 3.1.3.16; UniProt P67775; PP2A-alpha, PP2Ac, PP2Calpha, Replication protein C, RP-C) is encoded by the PPP2CA (also known as PP2CA) gene (Gene ID 5515) in human. Protein phosphatase 2 (PP2 or PP2A) is a ubiquitously expressed heterotrimeric serine/threonine protein phosphatase with broad substrate specificity, such as Raf, MEK, and AKT. PP2A is composed of a structural A and a catalytic C subunit as the dimeric core enzyme, and a regulatory B subunit. There exist two different A subunits (PPP2R1A & PPP2R1B), two different C subunits (PPP2CA & PPP2CB), and 12 different B subunits (PPP2R2B-D, PPP2R3A-C, PPP2R4, PPP2R5A-E). The enzymatic activity of a catalytic subunit is altered upon binding an A subunit even in the absence of a B subunit. The B subunits are believed to play key roles in controlling the localization and specific activity of the A/C holoenzymes.
      References
      Product Information
      FormatAlexaFluor®488
      PresentationPurified mouse monoclonal IgG2bκ antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-PP2A, C subunit Antibody, clone 1D6, Alexa Fluor® 488 Conjugate is an antibody against PP2A for use in Immunocytochemistry.
      Key Applications
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected PP2A in NIH/3T3 cells and HUVECs.
      The unconjugated antibody (Cat. No. 05-421) is shown to be suitable also for immunocytochemistry, immunoprecipitation, and Western blotting applications.
      Biological Information
      ImmunogenA 16 residue synthetic peptide (C-RGEPHVTRRTPDYFL) corresponding to amino acids 295-309 of the 36 kDa catalytic subunit of human protein phosphatase 2A (PP2A).
      EpitopeAmino acids 295-309.
      Clone1D6
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityExpected to recognize both spliced isoforms of human PP2A catalytic subunit reported by UniProt (P67775).
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      • Yeast
      • Xenopus
      • Bovine
      • Rat
      • Rabbit
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PPP2CA
      • PP2CA
      Purification MethodProtein A Purfied
      UniProt Number
      Molecular Weight~36 kDa observed. 35.59 kDa (isoform 1) and 29.74 kDa (isoform 2) calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in A431 cells.

      Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected PP2A in A431 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      05-421-AF488 04055977333657

      Documentation

      Anti-PP2A, C subunit Antibody, clone 1D6, Alexa Fluor® 488 Conjugate SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-PP2A, C subunit Antibody, clone 1D6, Alexa Fluor® 488 Conjugate Certificates of Analysis

      TitleLot Number
      Anti-PP2A, C subunit, clone 1D6, Alexa Fluor® 488 Conjugate - 3722072 3722072
      Anti-PP2A, C subunit, clone 1D6, Alexa Fluor® 488 Conjugate - 4067247 4067247
      Anti-PP2A, C subunit, clone 1D6, Alexa Fluor® 488 Conjugate - 4122587 4122587
      Anti-PP2A, C subunit, clone 1D6, Alexa Fluor® 488 Conjugate -Q2666772 Q2666772

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies