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219474 Cathepsin G Substrate IV, Fluorogenic - Calbiochem

219474
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219474-1MG
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      Description
      OverviewAn internally quenched fluorogenic peptide substrate (Mca/Anb as fluorophore/quencher) that is preferentially cleaved by cathepsin G between Gnf and Ser with high efficiency and selectivity (kcat = 0.524 s-1; KM = 2.08 µM; kcat/KM = 252,000 M-1s-1) as well as sensitivity (≤70 pM Cath G; [subsrate] = 3.3 µM). Human neutrophil elastase and proteinase 3 are found to cleave the peptide between Val and Thr with a much slower kinetics (no detectable cleavage in 1 h) and lower efficiency (10% and 23% cleavage, respectively, after 24 h). Excitation max: 325 nm; emission max: 400 nm.
      Mca = 7-Methoxycoumarin-4-yl acetyl; Gnf = 4-Guanidine-L-phenylalanine; Anb = Amino nitrobenzoic acid.
      Catalogue Number219474
      Brand Family Calbiochem®
      SynonymsMca-FVT-Gnf-SW-Anb-NH₂
      References
      ReferencesLesner, A., et al. 2008. Anal. Biochem. 375, 306.
      Product Information
      FormLyophilized solid
      FormulationSupplied as a trifluoroacetate salt
      Hill FormulaC₆₁H₆₇N₁₃O₁₅
      Chemical formulaC₆₁H₆₇N₁₃O₁₅
      Hygroscopic Hygroscopic
      Quality LevelMQ100
      Biological Information
      Purity≥97% by HPLC
      Physicochemical Information
      Emission max.
      Excitation max.
      Peptide SequenceMca-Phe-Val-Thr-Gnf~Ser-Trp-Anb-NH₂
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage -20°C
      Protect from Light Protect from light
      Hygroscopic Hygroscopic
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Global Trade Item Number
      Catalog Number GTIN
      219474-1MG 04055977218510