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PMEF-DR4 EmbryoMax® Primary Mouse Embryonic Fibroblasts

PMEF, Strain DR4, Mitomycin C Treated, Passage 3

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Stem Cell Culture
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PMEF-DR4
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      Key Specifications Table

      Key Applications
      Cult
      Description
      Catalogue NumberPMEF-DR4
      Trade Name
      • Embryomax
      • Specialty Media
      DescriptionEmbryoMax® Primary Mouse Embryonic Fibroblasts
      OverviewPlating MEF Feeder Cells

      Procedure:

      1. Prior to thawing PMEF feeder cells, coat plates/flasks with Gelatin solution.
      2. Thaw PMEF vial(s) quickly in a 37 °C water bath and transfer to a 15 mL tube (already containing 10 mL of warm PMEF Feeder Cell Medium). Gently invert the tube to distribute, and centrifuge at 300 xg for 4–5 minutes.
      3. Remove supernatant and resuspend the cell pellet in warm PMEF Feeder Cell Medium.
      4. Remove the Gelatin solution from plates/flasks, and aliquot the PMEF feeder cell suspension at the densities recommended in Table 4.1 of the mouse ES protocol guide
      5. Incubate the PMEF Feeder cells at 37 °C with 5% CO2. Use Figures 4A, B and C in the mouse ES protocol guide as a guide for an estimate of correct PMEF density and
      appearance. Gelatinized plates may be used for 12–14 days.
      Alternate Names
      • MEF Feeder Cells, DR4 PMEFs, DR4 MEFs, MEFs, Mouse Feeder Cells
      Background InformationThe EmbryoMax range of PMEF cells provides researchers with a convenient solution for ES cell culture by eliminating the need for time consuming feeder cell isolation and preparation. Many embryonic stem cell culture protocols necessitate the use of primary mouse embryo fibroblast (PMEF) cells. In these protocols, ES cells are typically cultured on a monolayer of PMEF feeder cells. Feeder cells perform two important roles in stem cell culture: they secrete several important growth factors into the medium, which help maintain pluripotency, and they provide a cellular matrix for ES cells to grow.

      The DR4 strain of MEF feeder cells are resistant to neomycin, hygromycin, puromycin, and 6-thioguanine. They are mitotically arrested by mytomycin-C treatment and will not proliferate.
      References
      Product Information
      Components
      • EmbryoMax PMEF, P3, Strain DR4, Mito-C Treated: (Part number PMEF-DR4) One (1) vial containing ≥4 x10^6 cells per vial.
      Quality LevelMQ100
      Applications
      ApplicationPMEF, Strain DR4, Mitomycin C Treated, Passage 3
      Key Applications
      • Stem Cell Culture
      Biological Information
      Cell Line Type
      • Primary Mouse Embryo Fibroblasts
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceCell Viability and Morphology: PASSED
      Mycoplasma Testing: PASSED
      Bacterial Testing: PASSED
      Fungi Testing: PASSED
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      Storage and Shipping Information
      Storage ConditionsVials should be stored in the vapor phase of liquid nitrogen.
      Packaging Information
      Material Size1 vial
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      PMEF-DR4 04054839159992