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MAB5268-K Mouse Anti-Chromogranin A, clone LK2H10 (2) (1mg)

Detect Chromogranin A using this Anti-Chromogranin A Antibody, clone LK2H10 validated for use in IH, IH(P) & WB.

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MAB5268-K
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      Description
      Catalogue NumberMAB5268-K
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionMouse Anti-Chromogranin A, clone LK2H10 (2) (1mg)
      OverviewThis product is intended for use by IVD manufacturers.
      Contact customer service for assistance: oem.reagents@emdmillipore.com
      Alternate Names
      • Anti-Chromogranin A
      Background InformationChromogranin A (a protein of 439 amino acids which is encoded on chromosome 14) is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, phenochromocytomas, paragangliomas, and other neuroendocrine tumors. Coexpression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms.
      References
      Product Information
      FormatPurified
      Control
      • Pancreas, adrenal gland, bowel and thyroid tissues Immunoblot: PC12 cell lysate, human pancreatic tissue
      PresentationPurified mouse monoclonal IgG1 in buffer containing 0.05M Borate buffered saline, 0.15M NaCl, pH 8.2 with 0.1% sodium azide.
      Quality LevelMQ300
      Applications
      ApplicationDetect Chromogranin A using this Anti-Chromogranin A Antibody, clone LK2H10 validated for use in IH, IH(P) & WB.
      Key Applications
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunohistochemistry:
      A 1-10 μg/mL concentration of a previous lot was used in IH.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenHuman pheochromocytoma
      CloneLK2H10
      Concentration1 .0 mg/mL
      HostMouse
      SpecificityChromogranin, an acidic protein having a molecular weight of about 68 kDa abides in the polypeptide-hormone-containing secretory granules of human endocrine tissues. Anti-chromogranin A can be used to stain most normal and neoplastic endocrine tissues containing secretory granules (exceptions include cells from the hypothalamus and posterior pituitary, which are known to contain secretory granules). The amount of staining is usually proportional to the number of secretory granules present (1).
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      • Monkey
      • Pig
      • Rat
      Species Reactivity NoteHuman, mouse, rat, monkey, and porcine. Does not recognize sheep, rabbit or guinea pig.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides; vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Other peptides, including chromostatin, beta-granin, WE-14 and GE-25, are also derived from the full-length protein. However, biological activities for these molecules have not been shown.
      Gene Symbol
      • CHGA
      • CgA
      • SP-I
      • CGA
      Purification MethodAmmonium Sulfate Precipitation
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P10645 # Pancreastatin strongly inhibits glucose induced insulin release from the pancreas.
      SIZE: 457 amino acids; 50730 Da
      SUBCELLULAR LOCATION: Secreted. Note=Neuroendocrine and endocrine secretory granules.
      PTM: Sulfated on tyrosine residues and/or contains sulfated glycans.
      SIMILARITY: SwissProt: P10645 ## Belongs to the chromogranin/secretogranin protein family.
      MISCELLANEOUS: Binds calcium with a low-affinity
      Molecular Weight50 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on Mouse Pancreas lysates.

      Western Blot Analysis:
      1:1000 dilution of this lot detected Chromogranin A on 10 μg of Mouse Pancreas lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 6 months at 2-8ºC in undiluted aliquots from date of receipt.
      Packaging Information
      Material SizeBulk packaging. Please inquire.
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MAB5268-K 04053252724114

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      Catalog Number Description  
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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies
      IVD/OEM Materials & Reagents > Bulk & Custom Antibodies > Primary Bulk Antibodies by Target > Tumor Markers & Antigens
      IVD/OEM Materials & Reagents > Bulk & Custom Antibodies > Primary Bulk Antibodies by Target > Neurological Proteins