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14-616M MSK2 Protein, active, 250 µg

Active, N-terminal His6-tagged, recombinant, human MSK2, amino acids 2-end, for use in Kinase Assays.

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14-616M
250 µg  Also available in 10 μg size (cat#14-616) and in bulk (cat#14-616-K).
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      Overview

      Replacement Information
      Description
      Catalogue Number14-616M
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionMSK2 Protein, active, 250 µg
      OverviewN-terminal His6-tagged, recombinant, human MSK2, amino acids 2-end
      References
      Product Information
      Quality LevelMQ300
      Applications
      ApplicationActive, N-terminal His6-tagged, recombinant, human MSK2, amino acids 2-end, for use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      Sourceexpressed by baculovirus in Sf21 insect cells
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including CREB1 and c-fos. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.
      Gene Symbol
      • RPS6KA4
      • MSK2
      • RSK-B
      • RSKB
      Protein TargetMSK2
      Purification MethodPurified using Ni2+/NTA-agarose. Activated using MAP Kinase 2/Erk2, active (Catalog # 14-173) and repurified using Ni2+/NTA-agarose
      Target Sub-FamilyAGC
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: O75676 # Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB. Essential role in the control of RELA transcriptional activity in response to TNF. Phosphorylates 'Ser- 10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF) and result in the transcriptional activation of several immediate early genes, including proto- oncogenes FOS and JUN (By similarity). Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 14 (HMG-14).
      COFACTOR: Magnesium.
      SIZE: 772 amino acids; 85606 Da
      SUBUNIT: Forms a complex with either ERK1 or ERK2 in quiescent cells which transiently dissociates following mitogenic stimulation. Also associates with MAPK14/p38-alpha. Activated RPS6KA4 associates with and phosphorylates the NF-kappa-B p65 subunit RELA.
      SUBCELLULAR LOCATION: Nucleus.
      SIMILARITY: SwissProt: O75676 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 2 protein kinase domains.
      MISCELLANEOUS: Enzyme activity requires the presence of both kinase domains (By similarity).
      Molecular Weight89.9kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by phosphorylation of 30mM Crosstide (Catalog # 12-331)
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions6 months at -70°C
      Packaging Information
      Material Size250 µg
      Material PackageAlso available in 10 μg size (cat#14-616) and in bulk (cat#14-616-K).
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      14-616M 04053252924330

      Documentation

      Related Products & Applications

      Alternative Packsize

      Catalog Number Description  
      14-616 MSK2 Protein, active, 10 µg Show Pricing & Availability

      Product Families

      Kinase & Phosphatase Products

      Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
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      Categories

      Life Science Research > Proteins and Enzymes > Purified Kinases
      Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases