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HTS088RTA Ready-to-Assay™ CysLT2 Leukotriene Receptor Frozen Cells

Human CysLT2 GPCR frozen cells for Calcium Flux FLIPR Assays.

Host Cell: Chem-1 Entrez Gene Number: NM_020377
HTS088RTA
1 kit  
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      Overview

      Replacement Information

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      This product requires a User Agreement (Label License). Please see product datasheet for further information

      Key Specifications Table

      SpeciesHost CellsProtein Target
      Human Chem-1CysLT2
      Description
      Catalogue NumberHTS088RTA
      Trade Name
      • Ready-to-Assay
      DescriptionReady-to-Assay™ CysLT2 Leukotriene Receptor Frozen Cells
      Background InformationMillipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

      The cysteinyl leukotrienes, leukotriene C4, leukotriene D4 and leukotriene E4, are arachidonic acid derivatives modified by glutathione, Cys-Gly or Cys. Activated mast cells release cysteinyl leukotrienes, which cause smooth muscle contraction, airway constriction, and vascular permeability. The biological effects of the cysteinyl leukotrienes are mediated by two GPCRs, CysLT1 and CysLT2. CysLT2 is expressed in moncytes/macrophages, mast cells and eosinophils. (Brink et al., 2003; Kanaoka and Boyce, 2004). A coding polymorphism in CysLT2 that results in a receptor with reduced response to LTD4 is associated with asthma (Pillai et al., 2004). Millipore’s cloned human CysLT2-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant CysLT2 expression on the cell surface for functional detection via the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at CysLT2 Receptor.
      References
      Product Information
      Components
      • Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
      • Fifty (50) mL of Media Component.
      Detection methodFluorescent
      Quality LevelMQ300
      Applications
      ApplicationHuman CysLT2 GPCR frozen cells for Calcium Flux FLIPR Assays.
      Key Applications
      • Calcium Flux Assays
      Biological Information
      Entrez Gene Number
      GPCR ClassA
      Host CellsChem-1
      Protein TargetCysLT2
      Target Sub-FamilyLeukotriene
      Cell Line Type
      • GPCR Cell Lines
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size1 kit
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      HTS088RTA 04053252481505

      Documentation

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      Categories

      Life Science Research > Cell Culture and Systems > Cell Lines > GPCR Frozen Cells
      Life Science Research > Drug Discovery and Development > GPCR Cell Lines & Assays > GPCR Frozen Cells