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MABS2267-100UL Anti-ANT1/SLC25A4 Antibody, clone IF3F11

Anti-ANT1/SLC25A4, clone IF3F11, Cat. No. MABS2267, is a mouse monoclonal antibody that detects ANT1/SLC25A4 and is tested for use in Western Blotting.

MABS2267-100UL
100 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABS2267-100UL
      DescriptionAnti-ANT1/SLC25A4 Antibody, clone IF3F11
      Alternate Names
      • ADP/ATP translocase 1
      • ADP/ATP carrier protein 1
      • ADP/ATP carrier protein
      • heart/skeletal muscle isoform T1
      • Adenine nucleotide translocator 1
      • Solute carrier family 25 member 4
      Background InformationADP/ATP translocase 1 (UniProt: P12235; also known as ADP/ATP carrier protein 1, ADP/ATP carrier protein, heart/skeletal muscle isoform T1 1 , Adenine nucleotide translocator 1, ANT 1, Solute carrier family 25 member 4) is encoded by the SLC25A4 (also known as AAC1, ANT1) gene (Gene ID: 291) in human. ANT1 is a multi-pass inner mitochondrial membrane protein with six transmembrane domains. It serves as an ADP:ATP antiporter that mediates import of ADP into the mitochondrial matrix for ATP synthesis, and export of ATP out to provide energy for cellular functions. It is reported to cycle between the cytoplasmic-open state (c-state) and the matrix-open state (m-state) and operates by the alternating access mechanism with a single substrate-binding site intermittently exposed to either the cytosolic or matrix side of the inner mitochondrial membrane. It is also involved in mitochondrial uncoupling and mitochondrial permeability transition pore activity. ANT also acts as a regulator of mitophagy independently of ADP:ATP antiporter activity. It promotes mitophagy via interaction with TIMM44 that leads to inhibition of presequence translocase TIMM23, thereby promoting stabilization of PINK1. ANT1 deficiency has been implicated in various pathological states, including hypertrophic cardiomyopathy, mitochondrial myopathy, lactic acidosis, and progressive external opthalmoplegia. (Ref.: Hoshino, A., et al. (2019). Nature. 575(7782); 375-379; Lu, Y-W., et al. (2017). Mol. Biol. Cell. 28(11):1489-1506; Kawamaoto, H., et al. (2011). Hum. Mol. Genet. 20(15); 2964-2974).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-ANT1/SLC25A4, clone IF3F11, Cat. No. MABS2267, is a mouse monoclonal antibody that detects ANT1/SLC25A4 and is tested for use in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesTested Applications

      Western Blotting Analysis: A 1:500 dilution from a representative lot detected ANT1/SLC25A4 in Human skeletal muscle and Human brain tissue extracts.

      Western Blotting Analysis: A representative lot detected ANT1/SLC25A4 in Western Blotting applications (Lu, Y-W., et al. (2017). Mol Biol Cell. 28(11):1489-1500; Acoba, M.G., et al. (2021). Cell Rep. 34(11):108869).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenHis-tagged, full-length, recombinant human ADP/ATP translocase 1 (ANT1).
      EpitopeInternal
      CloneIF3F11
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone IF3F11 is a mouse monoclonal antibody that detects Adenine nucleotide translocator 1 (ANT1/SLC25A4). It targets an epitope within 15 amino acids from the internal region.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SLC25A4
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~33 kDa observed; 33.06 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Human heart tissue lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected ANT1/SLC25A4 in Human heart tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABS2267-100UL 04065270321633