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MABC1216 Anti-ARHGAP36 Antibody, clone 12D10.1

Detect Arhgap36 using this mouse monoclonal Anti-ARHGAP36, clone 12D10.1 Antibody, Cat. No. MABC1216, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-ARHGAP36 Antibody, clone 12D10.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: ARHGAP36 UniProt Number: Q6ZRI8
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MABC1216
100 µL  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABC1216
      DescriptionAnti-ARHGAP36 Antibody, clone 12D10.1
      Alternate Names
      • Rho GTPase-activating protein 36
      Background InformationRho GTPase-activating protein 36 (UniProt Q6ZRI8) is encoded by the ARHGAP36 gene (Gene ID 158763) in human. ARHGAP36 is a Rho GAP family member that acts as a potent hedgehog (Hh) pathway positive regulator. ARHGAP36 is reported to functionally associate with Hh pathway negative regulator Sufu (suppressor of fused) and ARHGAP36 overexpression results in reduced Gli3R levels, Gli2 ciliary accumulation, and Gli1 expression, in a Smo-independent manner. ARHGAP36 is produced with a signal peptide sequence (a.a. 1-40), which is removed posttranslationally to yield the mature protein with a Rho-GAP domain (a.a. 226-426). Unlike other Rho GAP domain-containing proteins, ARHGAP36 lacks the arginine finger motif that stimulates GTPase hydrolysis and GAP domain mutants can retain Gli-activating activity. ARHGAP36 isoforms are up-regulated in a subset of medulloblastomas, where they mediate noncanonical Gli activation by inhibiting Sufu.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect Arhgap36 using this mouse monoclonal Anti-ARHGAP36, clone 12D10.1 Antibody, Cat. No. MABC1216, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected ARHGAP36 in human prostate cancer, adrenal gland and pancreas tissue sections.
      Biological Information
      ImmunogenGST-tagged recombinant human ARHGAP36 C-terminal fragment.
      Clone12D10.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 12D10.1 epitope lies within a C-terminal region present in all five human ARHGAP36 spliced isoforms reported by UniProt (Q6ZRI8).
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ARHGAP36
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight57.57/61.66 kDa (isoform 1 mature/proform), 57.57/58.34 kDa (isoform 2 mature/proform), 46.23 kDa (isoform 3), 57.57/60.57 kDa (isoform 4 mature/proform), 57.57 kDa (isoform 5).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Arhgap36 recombinant protein.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected 50 ng of GST-tagged recombinant human ARHGAP36 C-terminal fragment.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABC1216 04054839057243