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MAB5372 Anti-Astrocytomas Antibody, a marker for Low Grade Astrocytoma, clone J1-31

Detect Astrocytomas using this Anti-Astrocytomas Antibody, a marker for Low Grade Astrocytoma, clone J1-31 validated for use in WB, IC, IH(P).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Neuronal & Glial Markers
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MAB5372
100 µL  
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      Overview

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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Gp, H, M, RWB, ICC, IH(P)MAscitesMonoclonal Antibody
      Description
      Catalogue NumberMAB5372
      ReplacesMAB5314
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Astrocytomas Antibody, a marker for Low Grade Astrocytoma, clone J1-31
      References
      Product Information
      FormatAscites
      PresentationAscites fluid. Liquid.
      Quality LevelMQ100
      Applications
      ApplicationDetect Astrocytomas using this Anti-Astrocytomas Antibody, a marker for Low Grade Astrocytoma, clone J1-31 validated for use in WB, IC, IH(P).
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      Application NotesWestern blot: reducing conditions, recognizes a 30kDa protein {Singh, R et al (1986) Biosci Rep 6(1):73-80.

      Immunohistochemistry The antibody works on paraffin embedded tissue sections.

      Immunocytochemistry

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenHuman cerebral white matter plaque materials from a multiple sclerosis patient.
      Epitopea marker for Low Grade Astrocytoma
      CloneJ1-31
      HostMouse
      SpecificityThe antibody recognizes an intracellular protein antigen (MW 30 kDa) expressed by human and rat astrocytes and other specialized glia (Muller cells of the retina, Bergmann fibers of the cerebellar cortex, tanycytes of the hypothalamus and ciliated ependymal cells) in the central nervous system (CNS).The antibody has recently been found to be a specific marker for low grade astrocytoma in human brain tissue. The antibody is able to distinguish between low grade astrocytoma and normal reactive gliosis (patent application filed).

      Monoclonal antibody J1-31 was raised against crude homogenate of brain tissue from a multiple sclerosis (MS) patient (autopsy sample; Malhotra et al.: Microbios Letters 26:151-157, 1984). In human brain, MAb J1-31 recognizes an intracellular protein antigen (J1-31 antigen), which bands at approximately 30,000 daltons under reducing conditions for sodium dodecyl sulfate gel electrophoresis (Singh et al.: Bioscience Reports 6:73-79, 1986). By immunofluorescence microscopy, MAb J1-31 stains those cells that are also stained by antiserum to glial fibrillary acidic protein (GFAP), namely astrocytes, retinal Muller cells, and tanycytes in the ependyma (Predy et al.: Bioscience Reports 7:491-502, 1987). In addition, MAb J1-31 stains ciliated ependymal cells that do not express GFAP {Malhotra, SK (1989) J Neurosci Res. 22(1):36-49}.
      IsotypeIgM
      Species Reactivity
      • Guinea Pig
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MAB5372 04053252464003

      Documentation

      Anti-Astrocytomas Antibody, a marker for Low Grade Astrocytoma, clone J1-31 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Astrocytomas Antibody, a marker for Low Grade Astrocytoma, clone J1-31 Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-ASTROCYTES (MARKER FOR LOW GRADE ASTROCYTOMA) MONOCLONAL ANTIBODY 3088366
      MOUSE ANTI-ASTROCYTES (MARKER FOR LOW GRADE ASTROCYTOMA) MONOCLONAL ANTIBODY 2932383
      MOUSE ANTI-ASTROCYTES -2621528 2621528
      MOUSE ANTI-ASTROCYTES -2652173 2652173
      MOUSE ANTI-ASTROCYTES -2714467 2714467
      MOUSE ANTI-ASTROCYTES -2790072 2790072
      MOUSE ANTI-ASTROCYTES -2815178 2815178

      References

      Reference overviewPub Med ID
      Up-regulation of reactive astrogliosis in the rat glioma 9L cell line by combined mechanical and chemical injuries.
      Malhotra, S K, et al.
      Cytobios, 89: 115-34 (1997)  1997

      		9363621 9363621
      Diversity among reactive astrocytes: proximal reactive astrocytes in lacerated spinal cord preferentially react with monoclonal antibody J1-31.
      Malhotra, S K, et al.
      Brain Res. Bull., 30: 395-404 (1993)  1993

      		8457889 8457889
      Novel astrocytic protein in multiple sclerosis plaques.
      Malhotra, S K, et al.
      J. Neurosci. Res., 22: 36-49 (1989)  1989

      		2926840 2926840
      Enhanced expression of a protein antigen (J1-31 antigen, 30 kilodaltons) by reactive astrocytes in lacerated spinal cord.
      Predy, R, et al.
      J. Neurosci. Res., 19: 397-404, 466-7 (1988)  1988

      		3385801 3385801
      A new "marker" protein for astrocytes.
      Singh, R, et al.
      Biosci. Rep., 6: 73-80 (1986)  1986

      		2421799 2421799