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MAB1926-I Anti-Fibronectin Antibody, cell binding domain Antibody, clone P1H11

Anti-Fibronectin, cell binding domain, clone P1H11, Cat. No. MAB1926-I is a mouse monoclonal antibody that detects human fibronectin and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

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MAB1926-I
200 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMAB1926-I
      DescriptionAnti-Fibronectin Antibody, cell binding domain Antibody, clone P1H11
      Alternate Names
      • FN
      • Cold-insoluble globulin
      • CIG
      Background InformationFibronectin (UniProt: P02751; also known as FN, Cold-insoluble globulin, CIG) is encoded by the FN1 (also known as FN) gene (Gene ID: 2335) in human. Fibronectin is are involved various biological activities, including cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. It is essential for osteoblast mineralization and participates in the regulation of type I collagen deposition by osteoblasts. Fibronectin is usually present as homodimer of ~250 kDa subunits where subunits are connected by two disulfide bonds at the C-terminal region. Each monomer contains three repeating units, which are designated as type I, II, and III repeats containing about 40, 60, and 90 amino acids, respectively. Plasma fibronectin (soluble dimeric form) is secreted by hepatocytes and the cellular fibronectin (dimeric or cross-linked multimeric forms) is produced by fibroblasts, epithelial and other cell types. Cellular fibronectin is deposited as fibrils in the extracellular matrix. Fibronectin is recognized by integrins alpha5beta1 and alphaVbeta3. The primary sequence motif of fibronectin for integrin binding is a tripeptide, Arg-Gly-Asp (RGD). Mutations in FN gene are known to cause glomerulopathy that is characterized by proteinuria, microscopic hematuria, and hypertension, which can lead to end-stage renal failure.
      References
      Product Information
      FormatPurified
      HS Code3002 15 90
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Fibronectin, cell binding domain, clone P1H11, Cat. No. MAB1926-I is a mouse monoclonal antibody that detects human fibronectin and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      • Immunocytochemistry
      Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Fibronectin in human kidney and human cerebellum tissues.

      Western Blotting Analysis: A representative lot detected Fibronectin in Western Blotting applications (Zhang, H., et. al. (2010). Am J Physiol Renal Physiol. 299(1):F91-8).

      Immunocytochemistry Analysis: A representative lot detected Fibronectin in Immunocytochemistry applications (Mardilovich, A., et. al. (2006). Langmuir. 22(7):3259-64).

      Immunocytochemistry Analysis: A representative lot detected Fibronectin in Immunocytochemistry applications (Hofanson, D.M., et. al. (2010). Biomaterials. 31(26):6730-7).

      Immunocytochemistry Analysis: A representative lot detected Fibronectin in Immunocytochemistry applications (Waterman, R.S., et. al. (2010). PLoS One. 5(4):e10088).

      Immunohistochemistry Analysis: A representative lot detected Fibronectin in Immunohistochemistry applications (Carlos, C.P., et. al. (2014). PLoS One. 9(7):e103660).

      Western Blotting Analysis: A representative lot detected Fibronectin in Western Blotting applications (Zhang, H., et. al. (2008). Am J Physiol Renal Physiol. 295(4):F1071-81).
      Biological Information
      ImmunogenHuman Plasma Fibronectin.
      CloneP1H11
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone P1H11 detects fibronectin in human.
      IsotypeIgG1κ
      Species Reactivity
      • Mouse
      • Human
      • Porcine
      Species Reactivity NoteHuman, Mouse, Porcine.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • FN1
      • FN
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~270 kDa observed; 262.62 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human thymus tissue lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected Fibronectin in 10 µg of human thymus tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MAB1926-I 04054839337161

      Documentation

      Anti-Fibronectin Antibody, cell binding domain Antibody, clone P1H11 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Fibronectin Antibody, cell binding domain Antibody, clone P1H11 Certificates of Analysis

      TitleLot Number
      Anti-Fibronectin, cell binding domain, clone P1H11 - 3379916 3379916
      Anti-Fibronectin, cell binding domain, clone P1H11 - 3719553 3719553
      Anti-Fibronectin, cell binding domain, clone P1H11 - 3849497 3849497
      Anti-Fibronectin, cell binding domain, clone P1H11 Monoclonal Antibody 3107363

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