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MABF3113-100UG Anti-NKp30/CD337 Antibody, clone F252

Anti-NKp30/CD337, clone F252, Cat. No. MABF3113. is a mouse monoclonal antibody that detects -NKp30/CD337 and is tested for use in Flow Cytometry, Neutralizing, and Inhibition.

NewMABF3113-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF3113-100UG
      DescriptionAnti-NKp30/CD337 Antibody, clone F252
      Alternate Names
      • Natural cytotoxicity triggering receptor 3
      • Activating natural killer receptor p30
      • Natural killer cell p30-related protein
      • NK-p30
      • CD337
      Background InformationNatural cytotoxicity triggering receptor 3 (UniProt: O14931; also known as Activating natural killer receptor p30, Natural killer cell p30-related protein, NK-p30, NKp30, CD337) is encoded by the NCR3 (also known as 1C7, LY117) gene (Gene ID: 259197) in human. NKp30 is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-18), which is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 19-135), a transmembrane domain (aa 136-156), and a cytoplasmic domain (aa 157-201). It is selectively expressed by all resting and activated NK cells. Its weaker expression is observed in spleen. It serves as a receptor of NK cells. It is activated by binding of extracellular ligands including BAG6 and NCR3LG1 and stimulates NK cells cytotoxicity toward neighboring cells producing these ligands. It cooperates with NKp46 and/or NKp44 in inducing NK-mediated cytotoxicity against the majority of target cells. It is also reported to control NK cells cytotoxicity against tumor cells. Its interaction with BAG6 promotes myeloid dendritic cells (DC) maturation, both through killing DCs that fail to achieve a mature phenotype and by inducing the release of TNF- and interferon- by NK cells that promote DC maturation. Masking of NKp30 with specific monoclonal antibodies is reported to reduce maturation of DCs cocultured with NK cells. Addition of supernatant from NK cells stimulated via NKp30 is shown to induce DC maturation. Six isoforms of NKp30 have been described that are produced by alternative splicing. (Ref.: Vitale, M., et al. (2005). Blood. 106(2); 566-571; Pende, D., et al. (1999). J. Exp. Med. 190(10); 1505-1516).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgM in PBS without preservatives.
      Quality LevelMQ200
      Applications
      ApplicationAnti-NKp30/CD337, clone F252, Cat. No. MABF3113. is a mouse monoclonal antibody that detects -NKp30/CD337 and is tested for use in Flow Cytometry, Neutralizing, and Inhibition.
      Key Applications
      • Flow Cytometry
      • Affects Function
      • Neutralizing
      • Inhibition
      Application NotesTested Applications

      Flow Cytometry Analysis: A representative lot detected NKp30 (CD337) in Flow Cytometry application. (Meazza, R., et al. (2020). Cancers (Basel). 12(8):2187; Salome, B., et al. (2022). Cancer Cell. 40(9):1027-1043.e9).

      Inhibition: A representative lot Inhibited the lysis of antigen presenting cells (APC) and blocked the expression of CD83, CD86.(Spaggiari, G.M., et al. (2001). Eur J Immunol.;31(6):1656-65; Vitale, M., et al. (2005). Blood. 106(2):566-71).

      Flow Cytometry Analysis: A representative lot detected NKp30 (CD337) in Human peripheral blood mononuclear cells (PBMC). (Courtesy of Dr. Raffaella Meazza, IRCCS Ospedale Policlinico San Martino, Genova, Italy).

      Neutralizing: A representative lot neutralized the receptor function of NKp30 (CD337). (Witkowski, M., et al. (2021). Nature. 600(7888):295-301).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenActivated human natural killer (NK) cells.
      EpitopeExtracellular domain
      CloneF252
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone F252 is a mouse monoclonal antibody that detects Natural cytotoxicity triggering receptor 3 (NKp30/CD337). It targets an epitope within the extracellular domain.
      IsotypeIgMκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NCR3
      Purification MethodLigatrap
      UniProt Number
      Molecular Weight21.59 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in Human peripheral blood mononuclear cells (PBMC).

      Flow Cytometry Analysis: 1 µg of this antibody detected NKp30 in one million Human peripheral blood mononuclear cells (PBMC).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABF3113-100UG 04065271175129