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ABS217 Anti-phospho-LAT Antibody

Detect LAT using this rabbit polyclonal antibody, Anti-phospho-LAT Antibody validated for use in western blotting.

Anti-phospho-LAT Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Immunological Signaling Gene Symbol: LAT UniProt Number: O43561
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ABS217
100 µg  
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      Overview

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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABS217
      Replaces04-467
      DescriptionAnti-phospho-LAT Antibody
      Alternate Names
      • Linker for activation of T-cells family member 1
      • 36 kDa phospho-tyrosine adapter protein
      • pp36
      • p36-38
      Background InformationLAT (Linker for activation of T cells) is a transmembrane adaptor protein that plays an essential role in T cell receptor (TCR) signal transduction pathways. LAT is phosphorylated on multiple tyrosine residues upon TCR engagement, recruits several important signaling proteins to the membrane, and functions as a scaffold for the assembly of numerous signaling complexes.
      References
      Product Information
      FormatAffinity Purified
      Control
      • LAT immunoprecipitated from untreated or CD3 treated Jurkat cell lysates.
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect LAT using this rabbit polyclonal antibody, Anti-phospho-LAT Antibody validated for use in western blotting.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: 1 µg/mL from a representative lot detected phospho LAT (Tyr200) in CD3 treated Jurkat cell lysates. However, a decrease in signal was observed against phospho LAT (Tyr200) in untreated Jurkat cell lysates.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the cytoplasmic domain that interacts with GRB2, GRAP2 and PIK3R1 of human LAT phosphorylated at Tyrosine 200.
      EpitopeCytoplasmic domain that interacts with GRB2, GRAP2 and PIK3R1
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes the cytoplasmic domain that interacts with GRB2, GRAP2 and PIK3R1 of LAT phosphorylated at Tyrosine 200.
      Species Reactivity
      • Human
      Species Reactivity NoteDemonstrated to react with Human. Other homologies: Rat, Mouse, and Bovine (89% sequence homology).
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • LAT
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~36 kDa observed. Uncharacterized bands may be observed in some cell lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in LAT immunoprecipitated from untreated or CD3 treated Jurkat cell lysates.

      Western Blotting Analysis: 1 µg/mL of this antibody detected phospho LAT (Tyr200) immunoprecipitated from CD treated Jurkat cell lysates. A decrease in signal was observed against phospho LAT (Tyr200) immunoprecipitated from untreated Jurkat cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      ABS217 04053252906336

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies