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MABT1557-25UG Anti-Septin 7 Antibody, clone 19A4

Anti-Septin 7, clone 19A4, Cat. No. MABT1557, is a rat monoclonal antibody that detects Septin-7 and is tested for use in Immunoprecipitation and Western Blotting.

MABT1557-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABT1557-25UG
      DescriptionAnti-Septin 7 Antibody, clone 19A4
      Alternate Names
      • SEPT7
      • Septin-7
      Background InformationSeptin 7 (UniProt: F6UVF8; also known as SEPT7, Septin-7) is encoded by the SEPTIN7 gene (Gene ID: 100070492) in horse. Septins are highly conserved, GTP-binding cytoskeletal proteins that are involved in various cellular processes, including cytokinesis, membrane dynamics, cellular morphology, carcinogenesis, and neurodegeneration. Thus far 13 different septins have been reported in mammals. They can self-assemble and form filaments that serve as scaffolds for protein recruitment. Their assembly consists of hexamers and octamers that are formed by septin subunits, all including the essential group member Septin 7. Septin 7 can combine with other septins to form heteropolymers and it is a core component of these multimeric septin complexes. These heteropolymers can form a diverse array of higher order structures, including filaments, gauzes, and rings. Septin 7 is shown to be a novel regulator of neuronal calcium homoeostasis. It is widely distributed in the brain and is reported to be the most common septin in post-synaptic density fractions. It affects cytosolic calcium levels by down regulating the expression of the Orai and IP3 receptors. Lack of septin 7 can lead to loss of other septin proteins in homo- and heterooligomeric complexes. It is also shown to be essential for regulation of dendrite branching and dendritic spine morphology. Reduced levels of septin 7 have been reported in blood-derived lymphocytes in an equine model of recurrent uveitis (ERU) that affects inner eye and leads to vision loss. Dedicator of cytokinesis 8 (DOCK8) protein is shown to be an interaction partner of septin 7 in primary lymphocytes and its levels also show a significant decline in models of ERU. (Ref.: Wang, X., et al., (2018). J. Cell. Mol. Med. 22(7); 3298-3307; Schauer, M., et al. (2018). Sci. Rep. 8; 12332).
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG2c in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Septin 7, clone 19A4, Cat. No. MABT1557, is a rat monoclonal antibody that detects Septin-7 and is tested for use in Immunoprecipitation and Western Blotting.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation Analysis: A representative lot immunoprecipitated Septin-7 in Immunoprecipitation applications (Schauer, M., et. al. (2018). Sci Rep. 8(1):12332).

      Western Blotting Analysis: A representative lot detected Septin-7 in Western Blotting applications (Schauer, M., et. al. (2018). Sci Rep. 8(1):12332).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenA linear peptide corresponding to 19 amino acids from the C-terminal region of equine Septin 7.
      EpitopeC-terminus
      Clone19A4
      Concentration1 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRat
      SpecificityClone 19A4 is a rat monoclonal antibody that detects Septin 7 protein. It targets an epitope within 19 amino acids from the C-terminal region.
      IsotypeIgG2cκ
      Species Reactivity
      • Horse
      • Human
      Species Reactivity NoteHuman, Horse
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • SEPTIN7
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~48 kDa observed; 49.47 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human lymphocytes.

      Western Blotting Analysis: 1 µg/mL of this antibody detected Septin-7 in human lymphocytes.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABT1557-25UG 04061842070060

      Documentation

      Anti-Septin 7 Antibody, clone 19A4 Certificates of Analysis

      TitleLot Number
      Anti-Septin 7, clone 19A4 - Q3457400 Q3457400

      References

      Reference overviewPub Med ID
      Interaction of septin 7 and DOCK8 in equine lymphocytes reveals novel insights into signaling pathways associated with autoimmunity.
      Schauer M, Kleinwort KJH, Degroote RL, Wiedemann C, Kremmer E, Hauck SM, Deeg CA.
      Sci Rep  8(1)  12332  2018

      Show Abstract       		30120291 30120291