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KAA002 Sigma-AldrichCellular Senescence Assay

Cellular Senescence Assay Kits provide all the reagents required to efficiently detect SA-β-gal activity at pH 6.0 in cultured cells & tissue sections.

Cellular Senescence Assay MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

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KAA002

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Key Specifications Table

Detection Methods
Chromogenic

Description
Catalogue Number	KAA002
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Cellular Senescence Assay
Overview	Cellular senescence is one of the most fundamental aspects of cell behavior, and is thought to play a critical role in regulating cellular lifespan both in vitro and in vivo [1-3]. Primary somatic cells grown in vitro do not proliferate indefinitely. Instead, after a period of rapid proliferation, cell division rate slows, and ultimately ceases altogether, with the cells becoming unresponsive to mitogenic stimuli. This process is termed cellular senescence, and senescent cells have a well-defined accompanying phenotype - increased cell size, distinctive flat morphology, accumulated lipofuscin granules, wide changes in gene expression, and activity of senescence-associated β-galactosidase (SA-β-gal) [2,3]. It is generally believed that cellular senescence reflects some of the changes that occur during the aging of organisms, and senescent cells have been detected in vivo at sites of age-related pathology, such as benign hyperplastic prostate [4] and atherosclerotic lesions [5]. Recent studies have also provided convincing demonstrations of cellular senescence occurring in vivo in response to internally- and externally-induced stress signals [6,7]. In all of these studies, senescence was characterized by the appearance of senescence-associated β-galactosidase (SA-β-gal) activity, in common with the senescent phenotype in vitro. Cellular senescence has become an increasingly important target in the development of novel therapeutics. Emerging data implicates senescence bypass in the development of cancer and suggests that senescence may represent a tumor suppressor mechanism. The demonstration that tumor cells can be induced to undergo replicative senescence following the introduction of negative cell-cycle regulators, anti-telomerase peptides, or drug treatment suggests that induction of senescence can be exploited as a basis for cancer therapy [8,9]. For Research Use Only; Not for use in diagnostic procedures Test Principle: As outlined above, a classic characteristic of the senescent phenotype is the induction of senescence-associated β-galactosidase (SA-β-gal) activity. SA-β-gal is present only in senescent cells, not in presenescent, quiescent, or proliferating cells. Chemicon's Cellular Senescence Assay Kit provides all the reagents required to efficiently detect SA-β-gal activity at pH 6.0 in cultured cells and tissue sections. In this assay, SA-β-gal catalyzes the hydrolysis of X-gal, which results in the accumulation of a distinctive blue color in senescent cells. Each kit provides sufficient quantities of reagents to perform at least 50 assays in 35 mm wells.
Materials Required but Not Delivered	1. 1X PBS (Phosphate Buffered Saline) 2. 37°C incubator 3. Phase contrast or light microscope 4. Polypropylene tubes 5. Cells / Tissue samples 6. 70% glycerol (optional)

References

Product Information
Components	100X Fixing Solution: (Part No. 2004755) One 1.5 mL vial 10X Staining Solution A: (Part No. 2004756) One 15 mL bottle 10X Staining Solution B: (Part No. 2004754) One 15 mL bottle X-gal Solution: (Part No. 2004752) Two 1.5 mL vials
Detection method	Chromogenic
HS Code	3822 19 90
Quality Level	MQ100

Applications
Application	Cellular Senescence Assay Kits provide all the reagents required to efficiently detect SA-β-gal activity at pH 6.0 in cultured cells & tissue sections.

Biological Information

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Store X-gal solution protected from light at -20°C, and other kit components at 4°C. All components supplied are stable for 1 year. Precautions: Please refer to the Material Safety Data Sheet at www.chemicon.com for any necessary precautions.

Packaging Information
Material Size	1 kit

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalog Number	GTIN
KAA002	04053252009907

Documentation

Cellular Senescence Assay SDS

Title
Safety Data Sheet (SDS)

References

Reference overview	Pub Med ID
Telomere-independent cellular senescence in human fetal cardiomyocytes Ball, Andrew J and Levine, Fred Aging Cell, 4(1):21-30 (2005) 2005	15659210
What has senescence got to do with cancer? Dimri, Goberdhan P Cancer Cell, 7: 505-12 (2005) 2005 Show Abstract Cancer therapeutics are primarily thought to work by inducing apoptosis in tumor cells. However, various tumor suppressors and oncogenes have been shown to regulate senescence in normal cells, and senescence bypass appears to be an important step in the development of cancer. Cellular senescence limits the replicative capacity of cells, thus preventing the proliferation of cells that are at different stages of malignancy. A recent body of evidence suggests that induction of senescence can be exploited as a basis for cancer therapy.	15950900
Telomere shortening impairs organ regeneration by inhibiting cell cycle re-entry of a subpopulation of cells Satyanarayana, A, et al EMBO J, 22(15):4003-13 (2003) 2003	12881434
A senescence program controlled by p53 and p16INK4a contributes to the outcome of cancer therapy Schmitt, Clemens A, et al Cell, 109(3):335-46 (2002) 2002	12015983
Replicative senescence revisited. Marcotte, Richard and Wang, Eugenia J. Gerontol. A Biol. Sci. Med. Sci., 57: B257-69 (2002) 2002 Show Abstract Forty years after its discovery, replicative senescence remains a rich source of information about cell-cycle regulation and the progression from a normal to a transformed phenotype. Effectors of this growth-arrested state are being discovered at a great pace. This review discusses the latest findings on the players responsible for establishing replicative senescence, as well as the associated telomere shortening.	12084796
Differential expression of thymosin beta-10 by early passage and senescent vascular endothelium is modulated by VPF/VEGF: evidence for senescent endothelial cells in vivo at sites of atherosclerosis Vasile, E, et al FASEB J, 15(2):458-66 (2001) 2001	11156961
Expression of senescence-associated beta-galactosidase in enlarged prostates from men with benign prostatic hyperplasia Choi, J, et al Urology, 56(1):160-6 (2000) 2000	10869659
Senescence and immortalization of human cells Duncan, E L, et al Biogerontology, 1(2):103-21 (2000) 2000	11707927
A biomarker that identifies senescent human cells in culture and in aging skin in vivo Dimri, G P, et al Proc Natl Acad Sci USA, 92(20):9363-7 (1995) 1995	7568133

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Title
Cellular Senescence Assay Kit

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KAA002 Sigma-AldrichCellular Senescence Assay

Cellular Senescence Assay Kits provide all the reagents required to efficiently detect SA-β-gal activity at pH 6.0 in cultured cells & tissue sections.

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