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PMEF-H EmbryoMax® Primary Mouse Embryonic Fibroblasts

PMEF, Hygro Resistant, Strain C57/BL6, Mitomycin C Treated, Passage 3

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Stem Cell Culture
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PMEF-H
5 vials  5-6x106 ea
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      Overview

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      Key Specifications Table

      Key Applications
      Cult
      Description
      Catalogue NumberPMEF-H
      Trade Name
      • Embryomax
      • Specialty Media
      DescriptionEmbryoMax® Primary Mouse Embryonic Fibroblasts
      OverviewPrimary Mouse Embryo Fibroblasts, hygromycin resistant, strain C57/BL6, are Mytomycin C treated and serve as a feeder layer for both mouse and human ES/IPS cells. MEF cells are resistant to hygromycin.

      Plating MEF Feeder Cells

      Procedure:

      1. Prior to thawing PMEF feeder cells, coat plates/flasks with Gelatin solution.
      2. Thaw PMEF vial(s) quickly in a 37 °C water bath and transfer to a 15 mL tube (already containing 10 mL of warm PMEF Feeder Cell Medium). Gently invert the tube to distribute, and centrifuge at 300 xg for 4–5 minutes.
      3. Remove supernatant and resuspend the cell pellet in warm PMEF Feeder Cell Medium.
      4. Remove the Gelatin solution from plates/flasks, and aliquot the PMEF feeder cell suspension at the densities recommended in Table 4.1 of the mouse ES protocol guide
      5. Incubate the PMEF Feeder cells at 37 °C with 5% CO2. Use Figures 4A, B and C in the mouse ES protocol guide as a guide for an estimate of correct PMEF density and
      appearance. Gelatinized plates may be used for 12–14 days.
      Alternate Names
      • MEF Feeder Cells, hygro PMEFs, hygro MEFs, MEFs, Mouse Feeder Cells
      Background InformationThe EmbryoMax range of PMEF cells provides researchers with a convenient solution for ES cell culture by eliminating the need for time consuming feeder cell isolation and preparation. Many embryonic stem cell culture protocols necessitate the use of primary mouse embryo fibroblast (PMEF) cells. In these protocols, ES cells are typically cultured on a monolayer of PMEF feeder cells. Feeder cells perform two important roles in stem cell culture: they secrete several important growth factors into the medium, which help maintain pluripotency, and they provide a cellular matrix for ES cells to grow.
      References
      Product Information
      Quality LevelMQ100
      Applications
      ApplicationPMEF, Hygro Resistant, Strain C57/BL6, Mitomycin C Treated, Passage 3
      Key Applications
      • Stem Cell Culture
      Biological Information
      Stem Cell Type
      • Mouse Embryonic Stem Cells
      • PMEFs
      • Induced Pluripotent Stem Cells
      Cell Line Type
      • Primary Mouse Embryo Fibroblasts
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsAfter receipt, vials should be stored at -80°C. If storing for longer than 6 months, store in the vapor phase of liquid nitrogen (make sure the cap is tight) for up to 2 years.
      Packaging Information
      Material Size5 vials
      Material Package5-6x106 ea
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      PMEF-H 04053252613098

      Documentation

      Protocols

      Title
      General Experimental Outline for Targeting ES Cells
      Plating PMEF Feeder Cells Protocol

      EmbryoMax® Primary Mouse Embryonic Fibroblasts SDS

      Title

      Safety Data Sheet (SDS) 

      EmbryoMax® Primary Mouse Embryonic Fibroblasts Certificates of Analysis

      TitleLot Number
      EmbryoMax#174; PMEF Cells Hygro Resistant, Mito-C Treated - 2035339-1 2035339-1
      EmbryoMax#174; PMEF Cells Hygro Resistant, Mito-C Treated - 2021873 2021873
      EmbryoMax#174; PMEF Cells Hygro Resistant, Mito-C Treated - 2069003-1 2069003-1
      EmbryoMax#174; PMEF Cells Hygro Resistant, Mito-C Treated - 00510-5 00510-5
      EmbryoMax#174; PMEF Cells Hygro Resistant, Mito-C Treated - 00628-3 00628-3
      EmbryoMax#174; PMEF Cells Hygro Resistant, Mito-C Treated - 00811-3 00811-3
      EmbryoMax#174; PMEF Cells Hygro Resistant, Mito-C Treated - 01004-1 01004-1
      EmbryoMax#174; PMEF Cells Hygro Resistant, Mito-C Treated - 10119-6 10119-6
      EmbryoMax#174; PMEF Cells Hygro Resistant, Mito-C Treated - 10128-3 10128-3
      EmbryoMax#174; PMEF Cells Hygro Resistant, Mito-C Treated - 10407-2 10407-2
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      Brochure

      Title
      Murine Embryonic Stem Cell Culture

      Technical Info

      Title
      The Genetically Modified Mouse: An Indispensable Tool for Disease Modeling