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EQCYTMG-93KPX23 MILLIPLEX MAP Equine Cytokine/Chemokine Magnetic Bead Panel Premixed 23-Plex Panel - Immunology Multiplex Assay

The Equine Inflammation / Immunology Bead-Based Multiplex Cytokine Assay kit, using the Luminex xMAP technology, enables the simultaneous analysis of 23 cytokine and chemokine biomarkers in equine serum, plasma and cell culture samples.

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EQCYTMG-93KPX23
96-well plate  96 well plate
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      Overview

      Replacement Information
      Description
      Catalogue NumberEQCYTMG-93KPX23
      Trade Name
      • MILLIPLEX
      DescriptionMILLIPLEX MAP Equine Cytokine/Chemokine Magnetic Bead Panel Premixed 23-Plex Panel - Immunology Multiplex Assay
      OverviewEMD Millipore’s MILLIPLEX® MAP Equine Cytokine Magnetic Bead Premix Panel is a 23-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma or culture supernatant samples: Eotaxin/CCL11, FGF-2. Fractalkine/CS3CL1, G-CSF, GM-CSF, GRO, IFN, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8/CXCL8, IL-10, IL-12 (p70), IL-13, IL-17a, IL-18, IP-10, MCP-1, RANTES/CCL5 and TNFα.

      MILLIPLEX® MAP offers the broadest selection of analytes across a wide range of disease states and species. Once the analytes of interest have been identified, you can rely on the quality that we build into each kit to produce results you can trust. In addition to the assay characteristics listed in the protocol, other performance criteria evaluated during the validation process include: cross-reactivity, dilution linearity, kit stability, and sample behavior (e.g. detectability and stability). Each panel and kit meets stringent manufacturing criteria to ensure batch-to-batch reproducibility.

      Each MILLIPLEX® MAP panel and kit includes:
      • Quality controls (QCs) provided to qualify assay performance

      • Comparison of standard (calibrator) and QC lots to a reference lot to ensure lot-to-lot consistency

      • Optimized serum matrix to mimic native analyte environment

      • Detection antibody cocktails designed to yield consistent analyte profiles within panel


      EMD Millipore’s MILLIPLEX® MAP Equine Cytokine Magnetic Bead Panel is part of the most versatile system available for complement factor research. From our single to multiplex biomarker solutions, we partner with you to design, develop, analytically validate and build the most comprehensive library available for protein detection and quantitation.
      Background InformationInflammation and immune cell response are key to maintaining homeostasis. The more we understand the mechanisms of disease, the more we discover the role that inflammation plays in the etiology of disease. This is a true for horses as it is for humans. Both experience immunological diseases, such as infectious disease, osteoarthritis and respiratory disease, as well as neurologic, metabolic and cardiovascular disease and even cancer. However, until now the ability to measure levels of cytokines and chemokine in horses has been limited primarily to ELISAs. However, researchers will now the ability to measure multiple inflammation biomarkers simultaneously using EMD Millipore’s MILLIPLEX ® MAP Equine Cytokine/Chemokine Panel, based on the Luminex xMAP® technology.
      References
      Product Information
      Detection methodLuminex xMAP
      ConfigurationPremixed
      Precision, %
      • Inter-assay: <20
      • Intra-assay: <10
      Panel TypeMAGNETIC Cytokines/Chemokines
      Quality LevelMQ200
      Applications
      ApplicationThe Equine Inflammation / Immunology Bead-Based Multiplex Cytokine Assay kit, using the Luminex xMAP technology, enables the simultaneous analysis of 23 cytokine and chemokine biomarkers in equine serum, plasma and cell culture samples.
      Key Applications
      • Multiplexing
      Application Notes• This is an overnight (16-18 hours at 2-8 °C) or two-hour (20-25°C) incubation assay; although an overnight incubation is recommended.
      • A maximum of 25 µL per well of neat serum or plasma can be used. Tissue culture supernatant may require a dilution with an appropriate control medium prior to assay.
      • This kit may be used for the analysis of all of the Equine Cytokine/Chemokine Panel analytes in serum, plasma or tissue/cell/tumor culture supernatant.
      Biological Information
      SpecificityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
      Species Reactivity
      • Horse
      Analytes Available
      • Eotaxin/CCL11
      • FGF-2
      • Fractalkine/CX3CL1
      • G-CSF
      • GM-CSF
      • GRO/KC
      • IFN-γ
      • IL-1α
      • IL-1β
      • IL-2
      • IL-4
      • IL-5
      • IL-6
      • IL-8
      • IL-10
      • IL-12 (p70)
      • IL-13
      • IL-17A
      • IL-18
      • IP-10
      • MCP-1
      • RANTES
      • TNF-α
      Cross ReactivityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
      Accuracy
      • Eotaxin/CCL11: 93%
      • FGF-2: 78%
      • Fractalkine/CS3CL1: 91%
      • G-CSF: 82%
      • GM-CSF: 92%
      • GRO: 97%
      • IFNγ: 83%
      • IL-1α: 89%
      • IL-1β: 88%
      • IL-2: 90%
      • IL-4: 91%
      • IL-5: 92%
      • IL-6: 76%
      • IL-8/CXCL8: 89%
      • IL-10: 86%
      • IL-12 (p70): 83%
      • IL-13: 85%
      • IL-17a: 74%
      • IL-18: 94%
      • IP-10: 86%
      • MCP-1: 90%
      • RANTES/CCL5: 88%
      • TNFα: 87%
      Physicochemical Information
      Sensitivity
      • Eotaxin/CCL11: 8.3 pg/mL
      • FGF-2: 16.3 pg/mL
      • Fractalkine/CS3CL1: 457 pg/mL
      • G-CSF: 340 pg/mL
      • GM-CSF: 3.94 pg/mL
      • GRO: 7.8 pg/mL
      • IFNγ: 165 pg/mL
      • IL-1α: 39.1 pg/mL
      • IL-1β: 29.3 pg/mL
      • IL-2: 13.5 pg/mL
      • IL-4: 355 pg/mL
      • IL-5: 10.6 pg/mL
      • IL-6: 1.4 pg/mL
      • IL-8/CXCL8: 74.3 pg/mL
      • IL-10: 62.5 pg/mL
      • IL-12 (p70): 49.2 pg/mL
      • IL-13: 39 – 130 pg/mL
      • IL-17A: 15.4 pg/mL
      • IL-18: 25.3 pg/mL
      • IP-10: 16.7 pg/mL
      • MCP-1: 399 ng/mL
      • RANTES/CCL5: 3.9 pg/mL
      • TNFα: 5.2 pg/mL
      Standard Curve Range
      • Eotaxin/CCL11: 7 – 7,000 pg/mL
      • FGF-2: 24 – 25,000 pg/mL
      • Fractalkine/CS3CL1: 586 – 600,000 pg/mL
      • G-CSF: 78 – 80,000 pg/mL
      • GM-CSF: 5 – 5,000 pg/mL
      • GRO: 6 – 6,000 pg/mL
      • IFNγ: 488 – 500,000 pg/mL
      • IL-1α: 59 – 60,000 pg/mL
      • IL-1β: 29 – 30,000 pg/mL
      • IL-2: 15 – 15,000 pg/mL
      • IL-4: 293 – 300,000 pg/mL
      • IL-5: 15 – 15,000 pg/mL
      • IL-6: 15 – 15,000 pg/mL
      • IL-8/CXCL8: 59 – 60,000 pg/mL
      • IL-10: 49 – 50,000 pg/mL
      • IL-12 (p70): 39- 40,000 pg/mL
      • IL-13: 39 – 40,000 pg/mL
      • IL-17A: 15 – 15,000 pg/mL
      • IL-18: 34 – 35,000 pg/mL
      • IP-10: 15 – 15,000 pg/mL
      • MCP-1: 342 – 350,000 ng/mL
      • RANTES/CCL5: 5 – 5,000 pg/mL
      • TNFα: 4 - 4,000 pg/mL
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage for kit components is 2 - 8°C.
      Packaging Information
      Material Size96-well plate
      Material Package96 well plate
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      EQCYTMG-93KPX23 04055977350128

      Documentation

      Protocols

      Title
      Protocol- EQCYTMAG-93K

      MILLIPLEX MAP Equine Cytokine/Chemokine Magnetic Bead Panel Premixed 23-Plex Panel - Immunology Multiplex Assay SDS

      Title

      Safety Data Sheet (SDS) 

      Brochure

      Title
      A closer look at immunoassay platforms: Beyond fit for purpose
      Custom Immunoassay Menu of Services

      Technical Info

      Title
      96 well Template 2 Up
      96 well Template Single
      Quality Control Range Sheet- CYT-107 and CYT-207
      Quality Control Range Sheet- CYT-108 and CYT-208
      Quality Control Ranges: Milliplex® MAP Equine Cytokine/Chemokine Magnetic Bead Panel

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