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14-763M Tie2 (R849W) Protein, active, 250 µg

Active, N-terminal 6His-tagged recombinant human Tie2 residues 771-end containing the R849W substitution. For use in Kinase Assays.

14-763M
250 µg  Also available in 10 μg size (cat#14-763) and in bulk (cat# 14-763-K).
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      Overview

      Replacement Information
      Description
      Catalogue Number14-763M
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionTie2 (R849W) Protein, active, 250 µg
      OverviewN-terminal 6His-tagged recombinant human Tie2 residues 771-end containing the R849W substitution.
      Background InformationThe R849W mutation has been reported in dominantly inherited venous malformations. Expression studies in insect and mammalian cells demonstrates that Tie2 R849W has constitutively elevated tyrosine kinase activity compared with wild type. (Morris P.N., et al., Biochem.Biophys.Res.Commun.(2006);346:335-338 and Vikkula M, et al., Cell(1996);87:1181-1190).
      References
      Product Information
      Presentation50mM Tris/HCl pH8.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij 35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
      Quality LevelMQ300
      Applications
      ApplicationActive, N-terminal 6His-tagged recombinant human Tie2 residues 771-end containing the R849W substitution. For use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      SourceExpressed by baculovirus in Sf21 insect cell.
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Gene Symbol
      • TEK
      • TIE2
      Protein TargetTie2
      Purification MethodNi2+/NTA-agarose
      Target Sub-FamilyTK
      UniProt Number
      Molecular Weight42kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by phosphorylation of Poly (Glu4-Tyr) (4:1)
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore -70°C. For maximum recovery of product, centrifuge original vial prior to removing the cap.
      Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.
      Packaging Information
      Material Size250 µg
      Material PackageAlso available in 10 μg size (cat#14-763) and in bulk (cat# 14-763-K).
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      14-763M 04053252478925

      Documentation

      Related Products & Applications

      Alternative Packsize

      Catalog Number Description  
      14-763 Tie2 (R849W) Protein, active, 10 µg Show Pricing & Availability

      Product Families

      Kinase & Phosphatase Products

      Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
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      Categories

      Life Science Research > Proteins and Enzymes > Purified Kinases
      Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases