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14-925 Wee1 Protein, active, 10 µg

Active, N-terminal GST-tagged & C-terminal 6His tagged, recombinant, human Wee1, amino acids 214-end. For use in Kinase Assays.

Wee1 Protein, active, 10 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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14-925
10 µg  Also available in 250μg size as catalog # 14-925M and in bulk as catalog # 14-925-K.
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      Overview

      Replacement Information
      Description
      Catalogue Number14-925
      Trade Name
      • Upstate
      DescriptionWee1 Protein, active, 10 µg
      OverviewActs as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.
      References
      Product Information
      Presentation50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol.
      Quality LevelMQ100
      Applications
      ApplicationActive, N-terminal GST-tagged & C-terminal 6His tagged, recombinant, human Wee1, amino acids 214-end. For use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      SourceN-terminal GST-tagged and C-terminal 6His tagged, recombinant, human Wee1 amino acids 214-end expressed by baculovirus in Sf21 insect cells.
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Gene Symbol
      • WEE1
      Protein TargetWEE1
      Purification MethodPurified using glutathione agarose.
      Target Sub-FamilyCK1
      UniProt Number
      Molecular Weight77 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by phosphorylation of peptide LSNLYHQGKFLQTFCGSPLYRRR.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions6 months at -70°C
      Packaging Information
      Material Size10 µg
      Material PackageAlso available in 250μg size as catalog # 14-925M and in bulk as catalog # 14-925-K.
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      14-925 04053252832147

      Related Products & Applications

      Alternative Packsize

      Catalog Number Description  
      14-925M Wee1 Protein, active, 250 µg Show Pricing & Availability

      Product Families

      Kinase & Phosphatase Products

      Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
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      Categories

      Life Science Research > Proteins and Enzymes > Purified Kinases
      Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases